- Featured Product
- KD/KO Validated
BCL2 Monoclonal antibody, PBS Only (Capture)
BCL2 Monoclonal Antibody for IHC, IF/ICC, IF-P, IP, Cytometric bead array, Indirect ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, pig
Applications
IHC, IF/ICC, IF-P, IP, Cytometric bead array, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
4H8C6
验证数据展示
产品信息
60178-1-PBS targets BCL2 as part of a matched antibody pair:
MP50290-2: 60178-1-PBS capture and 60178-2-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
Tested Applications | IHC, IF/ICC, IF-P, IP, Cytometric bead array, Indirect ELISA Application Description |
Tested Reactivity | human, pig |
Immunogen | BCL2 fusion protein Ag3508 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | B-cell CLL/lymphoma 2 |
Synonyms | BCL-2, 4H8C6 |
Calculated Molecular Weight | 26 kDa |
Observed Molecular Weight | 26 kDa |
GenBank Accession Number | BC027258 |
Gene Symbol | BCL2 |
Gene ID (NCBI) | 596 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P10415 |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
1) What is Bcl-2?
Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.2) FAQs for Bcl-2
A) How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
B) What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.