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验证数据展示

  • IHC staining of human appendicitis using 60178-1-Ig (same clone as 60178-1-PBS)

    IHC staining of human appendicitis using 60178-1-Ig (same clone as 60178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2 antibody included
  • IHC staining of human appendicitis using 60178-1-Ig (same clone as 60178-1-PBS)

    IHC staining of human appendicitis using 60178-1-Ig (same clone as 60178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2 antibody included
  • IF Staining of MCF-7 using 60178-1-Ig (same clone as 60178-1-PBS)

    IF Staining of MCF-7 using 60178-1-Ig (same clone as 60178-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using BCL2 antibody (60178-1-Ig, Clone: 4H8C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

  • IP experiment of U-937 using 60178-1-Ig (same clone as 60178-1-PBS)

    IP experiment of U-937 using 60178-1-Ig (same clone as 60178-1-PBS)

    IP result of anti-BCL2 (IP:60178-1-Ig, 5ug; Detection:60178-1-Ig 1:1000) with U-937 cells lysate 4000ug. This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60178-1-Ig (same clone as 60178-1-PBS)

    IHC staining of human tonsillitis using 60178-1-Ig (same clone as 60178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2 antibody included
  • IF Staining of human appendicitis using 60178-1-Ig (same clone as 60178-1-PBS)

    IF Staining of human appendicitis using 60178-1-Ig (same clone as 60178-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using BCL2 antibody (60178-1-Ig, Clone: 4H8C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50290-2

    Cytometric bead array standard curve of MP50290-2

    Cytometric bead array standard curve of MP50290-2, BCL2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60178-1-PBS. Detection antibody: 60178-2-PBS. Standard:Ag3508. Range: 1.563-100 ng/mL.

  • Featured Product
  • KD/KO验证

BCL2 Monoclonal antibody, PBS Only (Capture)

BCL2 Monoclonal Antibody for IHC, IF/ICC, IF-P, IP, Cytometric bead array, Indirect ELISA
Cat No. 60178-1-PBS

产品说明书

CloneNo. 4H8C6

宿主/亚型

Mouse / IgG2b

种属反应性

human, pig

应用

IHC, IF/ICC, IF-P, IP, Cytometric bead array, Indirect ELISA

别名

BCL-2, 4H8C6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy BCL2 Ready-To-Use IHC Kit. BCL2 primary antibody included.

产品信息

60178-1-PBS targets BCL2 as part of a matched antibody pair:

MP50290-2: 60178-1-PBS capture and 60178-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IHC, IF/ICC, IF-P, IP, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原 BCL2 fusion protein Ag3508 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 B-cell CLL/lymphoma 2
别名 BCL-2, 4H8C6
计算分子量 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC027258
基因名称 BCL2
Gene ID (NCBI) 596
RRIDAB_10734459
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP10415
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

1) What is Bcl-2?
Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.
2) FAQs for Bcl-2

A) How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
B) What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.