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  • Featured Product
  • KD/KO Validated

BMI1 Monoclonal antibody, PBS Only

BMI1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, monkey

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2E10A11

Cat no : 66161-1-PBS

Print datasheet

Synonyms

RNF51, Polycomb group RING finger protein 4, Polycomb complex protein BMI-1, PCGF4, 2E10A11

验证数据展示

  • WB analysis of PC-12 using 66161-1-Ig (same clone as 66161-1-PBS)

    WB analysis of PC-12 using 66161-1-Ig (same clone as 66161-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 66161-1-Ig (same clone as 66161-1-PBS)

    IP experiment of HEK-293 using 66161-1-Ig (same clone as 66161-1-PBS)

    IP result of anti-BMI1 (IP:66161-1-Ig, 3ug; Detection:66161-1-Ig 1:1000) with HEK-293 cells lysate 1800ug. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 66161-1-Ig (same clone as 66161-1-PBS)

    IHC staining of human lymphoma using 66161-1-Ig (same clone as 66161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66161-1-Ig (BMI1 Antibody) at dilution of 1:50 under 40x lens. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BMI1 antibody included
  • IHC staining of human breast cancer using 66161-1-Ig (same clone as 66161-1-PBS)

    IHC staining of human breast cancer using 66161-1-Ig (same clone as 66161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66161-1-Ig (BMI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BMI1 antibody included
  • IF Staining of HEK-293 using 66161-1-Ig (same clone as 66161-1-PBS)

    IF Staining of HEK-293 using 66161-1-Ig (same clone as 66161-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using BMI1 antibody (66161-1-Ig, Clone: 2E10A11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy BMI1 Ready-To-Use IHC Kit. BMI1 primary antibody included.

产品信息

66161-1-PBS targets BMI1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, monkey
Immunogen BMI1 fusion protein Ag21284 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name BMI1 polycomb ring finger oncogene
Synonyms RNF51, Polycomb group RING finger protein 4, Polycomb complex protein BMI-1, PCGF4, 2E10A11
Calculated Molecular Weight 37 kDa
Observed Molecular Weight37-43 kDa
GenBank Accession NumberBC011652
Gene Symbol BMI1
Gene ID (NCBI) 648
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35226
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody reacts with the 37kd BMI1 protein.