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ATG14/Barkor (N-terminal) Polyclonal antibody

ATG14/Barkor (N-terminal) Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

IF, IHC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 19491-1-AP

Print datasheet

Synonyms

ATG14, Atg14L, Barkor, KIAA0831



经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells, mouse brain tissue, NIH/3T3 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19491-1-AP targets ATG14/Barkor (N-terminal) in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF, IHC, WB,ELISA
Cited Applications IF, IP, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, canine, bovine
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIAA0831
Synonyms ATG14, Atg14L, Barkor, KIAA0831
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 65-68 kDa
GenBank Accession NumberNM_014924
Gene Symbol KIAA0831
Gene ID (NCBI) 22863
RRIDAB_10642701
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZNE5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Barkor, also named as KIAA0831, Atg14 and Atg14L, is required for both basal and inducible autophagy. It plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Barkor promotes BECN1 translocation from the trans-Golgi network to autophagosomes. This antibody is specific to KIAA0831(Barkor).

实验方案

Product Specific Protocols
WB protocol for ATG14/Barkor (N-terminal) antibody 19491-1-APDownload protocol
IHC protocol for ATG14/Barkor (N-terminal) antibody 19491-1-APDownload protocol
IF protocol for ATG14/Barkor (N-terminal) antibody 19491-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy

Authors - Yang Meng
humanWB

Mol Cancer

PMID: 32727463

Authors - Cefan Zhou
humanWB

Adv Sci (Weinh)

Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia

Authors - Tiemei Li
humanWB

Autophagy

STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.

Authors - Cefan Zhou
mouseWB

Dev Cell

Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.

Authors - Yuqing Lei
humanWB

Autophagy

Simultaneous inhibition of the ubiquitin-proteasome system and autophagy enhances apoptosis induced by ER stress aggravators in human pancreatic cancer cells.

Authors - Xu Li