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Bcl-XL Monoclonal antibody, PBS Only (Detector)

Bcl-XL Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4C12A6

Cat no : 66020-1-PBS

Print datasheet

Synonyms

BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, Apoptosis regulator Bcl X

验证数据展示

  • WB analysis of Jurkat using 66020-1-Ig (same clone as 66020-1-PBS)

    WB analysis of Jurkat using 66020-1-Ig (same clone as 66020-1-PBS)

    Jurkat, Ramos, K-562 and Raw 264.7 cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66020-1-Ig (same clone as 66020-1-PBS)

    IHC staining of human breast cancer using 66020-1-Ig (same clone as 66020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2L1 antibody included
  • IHC staining of human breast cancer using 66020-1-Ig (same clone as 66020-1-PBS)

    IHC staining of human breast cancer using 66020-1-Ig (same clone as 66020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2L1 antibody included
  • FC experiment of HeLa using 66020-1-Ig (same clone as 66020-1-PBS)

    FC experiment of HeLa using 66020-1-Ig (same clone as 66020-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Bcl-XL (66020-1-Ig, Clone:4C12A6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66020-1-Ig (same clone as 66020-1-PBS)

    IF Staining of HeLa using 66020-1-Ig (same clone as 66020-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Bcl-XL antibody (66020-1-Ig, Clone: 4C12A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50613-1

    Cytometric bead array standard curve of MP50613-1

    Cytometric bead array standard curve of MP50613-1, Bcl-xL Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66020-2-PBS. Detection antibody: 66020-1-PBS. Standard:Ag18037. Range: 1.563-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy BCL2L1 Ready-To-Use IHC Kit. BCL2L1 primary antibody included.

产品信息

66020-1-PBS targets Bcl-XL as part of a matched antibody pair:

MP50613-1: 66020-2-PBS capture and 66020-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Bcl-XL fusion protein Ag18037 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name BCL2-like 1
Synonyms BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, Apoptosis regulator Bcl X
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC019307
Gene Symbol Bcl-XL
Gene ID (NCBI) 598
RRIDAB_11042315
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07817
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.