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验证数据展示

  • WB analysis of human plasma using 66268-1-Ig (same clone as 66268-1-PBS)

    WB analysis of human plasma using 66268-1-Ig (same clone as 66268-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66268-1-Ig (C1QC Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66268-1-PBS in a different storage buffer formulation.

  • IF Staining of HUVEC using 66268-1-Ig (same clone as 66268-1-PBS)

    IF Staining of HUVEC using 66268-1-Ig (same clone as 66268-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using C1qC antibody (66268-1-Ig, Clone: 1C5B4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66268-1-PBS in a different storage buffer formulation.

C1qC Monoclonal antibody, PBS Only

C1qC Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66268-1-PBS

产品说明书

CloneNo. 1C5B4

宿主/亚型

Mouse / IgM

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

Complement C1q C chain, C1Q C, 1C5B4, C1QG

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy C1QC Ready-To-Use IHC Kit. C1QC primary antibody included.

产品信息

66268-1-PBS targets C1qC in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10233

Product name: Recombinant human C1QC protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 27-245 aa of BC009016

Sequence: QANTGCYGIPGMPGLPGAPGKDGYDGLPGPKGEPGIPAIPGIRGPKGQKGEPGLPGHPGKNGPMGPPGMPGVPGPMGIPGEPGEEGRYKQKFQSVFTVTRQTHQPPAPNSLIRFNAVLTNPQGDYDTSTGKFTCKVPGLYYFVYHASHTANLCVLLYRSGVKVVTFCGHTSKTNQVNSGGVLLRLQVGEEVWLAVNDYYDMVGIQGSDSVFSGFLLFPD

种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 complement component 1, q subcomponent, C chain
别名 Complement C1q C chain, C1Q C, 1C5B4, C1QG
计算分子量 245 aa, 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC009016
基因名称 C1QC
Gene ID (NCBI) 714
RRIDAB_2881653
偶联类型 Unconjugated
形式Liquid
纯化方式Thiophilic affinity chromatograph
UNIPROT IDP02747
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qC which is the C-chain polypeptide of human complement subcomponent C1q.

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