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C1qC Monoclonal antibody, PBS Only

C1qC Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C5B4

Cat no : 66268-1-PBS

Print datasheet

Synonyms

C1Q C, C1qC, C1QG

验证数据展示

  • WB analysis of human plasma using 66268-1-Ig (same clone as 66268-1-PBS)

    WB analysis of human plasma using 66268-1-Ig (same clone as 66268-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66268-1-Ig (C1QC Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66268-1-PBS in a different storage buffer formulation.

  • IF Staining of HUVEC using 66268-1-Ig (same clone as 66268-1-PBS)

    IF Staining of HUVEC using 66268-1-Ig (same clone as 66268-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using C1qC antibody (66268-1-Ig, Clone: 1C5B4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66268-1-PBS in a different storage buffer formulation.

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy C1QC Ready-To-Use IHC Kit. C1QC primary antibody included.

产品信息

66268-1-PBS targets C1qC in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen C1qC fusion protein Ag10233 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name complement component 1, q subcomponent, C chain
Synonyms C1Q C, C1qC, C1QG
Calculated Molecular Weight 245 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC009016
Gene Symbol C1QC
Gene ID (NCBI) 714
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDP02747
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qC which is the C-chain polypeptide of human complement subcomponent C1q.