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  • KD/KO Validated

cGAS Polyclonal antibody

cGAS Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 26416-1-AP

Print datasheet

Synonyms

C6orf150, cGAS, Cyclic GMP-AMP synthase, h-cGAS, MB21D1



经过测试的应用

Positive WB detected inHL-60 cells, THP-1 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26416-1-AP targets cGAS in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IF, IHC, IP, WB
Tested Reactivity human
Cited Reactivityhuman, pig
Immunogen cGAS fusion protein Ag23455 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 6 open reading frame 150
Synonyms C6orf150, cGAS, Cyclic GMP-AMP synthase, h-cGAS, MB21D1
Calculated Molecular Weight 496 aa, 54 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC113608
Gene Symbol C6orf150
Gene ID (NCBI) 115004
RRIDAB_2880507
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N884
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.

实验方案

Product Specific Protocols
WB protocol for cGAS antibody 26416-1-APDownload protocol
IHC protocol for cGAS antibody 26416-1-APDownload protocol
IF protocol for cGAS antibody 26416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion

Authors - Wenwen Zheng
humanWB,IF

Cell

Poxviruses Evade Cytosolic Sensing through Disruption of an mTORC1-mTORC2 Regulatory Circuit.

Authors - Nathan Meade
WB,IF

Adv Sci (Weinh)

PICH Supports Embryonic Hematopoiesis by Suppressing a cGAS-STING-Mediated Interferon Response.

Authors - Xinwei Geng
humanWB

Nat Commun

ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling

Authors - Maximilian Hirschenberger
WB

Mol Cell

CPT1A induction following epigenetic perturbation promotes MAVS palmitoylation and activation to potentiate antitumor immunity

Authors - Guiheng Zhang
  • KO Validated
humanWB

J Clin Invest

Melatonin inhibits cytosolic mitochondrial DNA-induced neuroinflammatory signaling in accelerated aging and neurodegeneration.

Authors - Abhishek Jauhari