验证数据展示
经过测试的应用
| Positive WB detected in | human stomach tissue, HL-60 cells, mouse small intestine tissue |
| Positive IHC detected in | human stomach cancer tissue, human kidney tissue, human lung tissue, human ovary tissue, human stomach tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17556-1-AP targets Calpain 9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Calpain 9 fusion protein Ag11718 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | calpain 9 |
| 别名 | NCL4, GC36, EC:3.4.22.-, Digestive tract-specific calpain, CAPN9 |
| 计算分子量 | 627 aa, 72 kDa |
| 观测分子量 | 60-66 kDa |
| GenBank蛋白编号 | BC027993 |
| 基因名称 | Calpain 9 |
| Gene ID (NCBI) | 10753 |
| RRID | AB_2290833 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14815 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Calpain 9 is also named as NCL4 and belongs to the peptidase C2 family.It can be down-regulated in gastric cancer tissue and in gastric cell lines of differentiated and poorly differentiated types.It is predominantly expressed in the gastrointestinal tract and restricted to the gastric surface mucus (pit) cells in the stomach and the function of it is implicated in suppressing tumorigenesis(PMID:20686710).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Calpain 9 antibody 17556-1-AP | Download protocol |
| IHC protocol for Calpain 9 antibody 17556-1-AP | Download protocol |
| IF protocol for Calpain 9 antibody 17556-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
