Calpain 9 Polyclonal antibody
Calpain 9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human stomach tissue, HL-60 cells, mouse small intestine tissue |
| Positive IHC detected in | human stomach cancer tissue, human kidney tissue, human lung tissue, human ovary tissue, human stomach tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17556-1-AP targets Calpain 9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Calpain 9 fusion protein Ag11718 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | calpain 9 |
| Synonyms | NCL4, GC36, EC:3.4.22.-, Digestive tract-specific calpain, CAPN9 |
| Calculated Molecular Weight | 627 aa, 72 kDa |
| Observed Molecular Weight | 60-66 kDa |
| GenBank Accession Number | BC027993 |
| Gene Symbol | Calpain 9 |
| Gene ID (NCBI) | 10753 |
| RRID | AB_2290833 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14815 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Calpain 9 is also named as NCL4 and belongs to the peptidase C2 family.It can be down-regulated in gastric cancer tissue and in gastric cell lines of differentiated and poorly differentiated types.It is predominantly expressed in the gastrointestinal tract and restricted to the gastric surface mucus (pit) cells in the stomach and the function of it is implicated in suppressing tumorigenesis(PMID:20686710).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Calpain 9 antibody 17556-1-AP | Download protocol |
| IHC protocol for Calpain 9 antibody 17556-1-AP | Download protocol |
| IF protocol for Calpain 9 antibody 17556-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Pharmacol Inhibition of calpain9 attenuates peritoneal dialysis-related peritoneal fibrosis | ||
Sci Rep Decreased expression of Calpain-9 predicts unfavorable prognosis in patients with gastric cancer. |
