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CAPZA1 Polyclonal antibody
CAPZA1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue |
| Positive IP detected in | SH-SY5Y cells |
| Positive IHC detected in | human ovary tumor tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CAPZA1 Ready-To-Use IHC Kit. CAPZA1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11806-1-AP targets CAPZA1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IP, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | CAPZA1 fusion protein Ag2267 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | capping protein (actin filament) muscle Z-line, alpha 1 |
| Synonyms | CAPPA1, CAPZ, CapZ alpha 1, CAPZA1, CAZ1 |
| Calculated Molecular Weight | 286 aa, 33 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC000144 |
| Gene Symbol | CAPZA1 |
| Gene ID (NCBI) | 829 |
| RRID | AB_2070037 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P52907 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CAPZA1 (F-actin-capping protein subunit alpha-1) is a member of the F-actin capping protein alpha subunit family and regulates actin filament growth, stability and disassembly. Differential expression of CAPZA1 has been found in gastric cancer and melanoma, indicating its possible role in tumorigenesis. (21566537, 23545944)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CAPZA1 antibody 11806-1-AP | Download protocol |
| IHC protocol for CAPZA1 antibody 11806-1-AP | Download protocol |
| IP protocol for CAPZA1 antibody 11806-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol Heterodimeric capping protein is required for stereocilia length and width regulation. | ||
J Exp Clin Cancer Res CAPZA1 modulates EMT by regulating actin cytoskeleton remodelling in hepatocellular carcinoma.
| ||
Invest Ophthalmol Vis Sci Differential expression of fourteen proteins between uveal melanoma from patients who subsequently developed distant metastases versus those who did Not. | ||
Melanoma Res Differential expression patterns of capping protein, protein phosphatase 1, and casein kinase 1 may serve as diagnostic markers for malignant melanoma. |
