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Caspase 1/P20 Polyclonal antibody

Caspase 1/P20 Polyclonal Antibody for WB, IHC, IF, ELISA
Cat No. 22915-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (6)

应用

WB, IHC, IF, ELISA and More (2)

CASP1, Caspase1, CASP 1, CASP-1, Caspase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inRaji cells, HepG2 cells, Jurkat cells, THP-1 cells, U-937 cells, PC-12 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22915-1-AP targets Caspase 1/P20 in WB, IHC, IF, IP, ELISA, IHC-IF applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF, ELISA Application Description
文献引用应用WB, IHC, IF, IP, IHC-IF
经测试反应性 human
文献引用反应性human, pig, canine, monkey, chicken, bovine, hamster
免疫原 Caspase 1/P20 fusion protein Ag11143 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
别名 CASP1, Caspase1, CASP 1, CASP-1, Caspase 1
计算分子量 404 aa, 45 kDa
观测分子量 45-47 kDa, 30 kDa, 35 kDa
GenBank蛋白编号BC062327
基因名称 Caspase 1
Gene ID (NCBI) 834
RRIDAB_2876874
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP29466
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 22915-1-AP can recognize p45 procaspase and the mature fragment p20.

实验方案

Product Specific Protocols
WB protocol for Caspase 1/P20 antibody 22915-1-APDownload protocol
IHC protocol for Caspase 1/P20 antibody 22915-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Mol Cancer

Metal-enriched HSP90 nanoinhibitor overcomes heat resistance in hyperthermic intraperitoneal chemotherapy used for peritoneal metastases

Authors - Qiang Wang
humanWB

ACS Nano

Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.

Authors - Zhiwei Liao
WB,IF

Mol Cell

Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation

Authors - Chuanlong Wang
IHC

J Hazard Mater

Perinatal exposure to PBEB aggravates liver injury via macrophage-derived TWEAK in male adult offspring mice under western diet

Authors - Wanyue Wang
humanWB

J Hazard Mater

Adverse outcome pathway-based approach to reveal the mechanisms of skin sensitization and long-term aging effects of chlorothalonil

Authors - Yung-Hsuan Cheng
WB

Nat Commun

REV-ERBα integrates colon clock with experimental colitis through regulation of NF-κB/NLRP3 axis.

Authors - Shuai Wang
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