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Caspase 3/p17/p19 Monoclonal antibody

Caspase 3/p17/p19 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (6)

Applications

IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2G4B2

Cat No : 66470-2-Ig

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Synonyms

Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17/p19, Caspase3, Caspase-3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1



经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, HepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, mouse liver tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66470-2-Ig targets Caspase 3/p17/p19 in WB, IHC, IF, ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications ICC, IF, IHC, WB
Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse, rat, chicken, pig, plant, canine
Immunogen Caspase 3/p17/p19 fusion protein Ag25029 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name caspase 3, apoptosis-related cysteine peptidase
Synonyms Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17/p19, Caspase3, Caspase-3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1
Calculated Molecular Weight 277 aa, 32 kDa
Observed Molecular Weight 32-35 kDa, 19 kDa, 17 kDa
GenBank Accession NumberBC016926
Gene Symbol CASP3
Gene ID (NCBI) 836
RRIDAB_2876892
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP42574
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

实验方案

Product Specific Protocols
WB protocol for Caspase 3/p17/p19 antibody 66470-2-IgDownload protocol
IHC protocol for Caspase 3/p17/p19 antibody 66470-2-IgDownload protocol
IF protocol for Caspase 3/p17/p19 antibody 66470-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,ICC

Acta Pharm Sin B

Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.

Authors - Xunxun Wu
humanWB

Small

Inherent Capability of Self-Assembling Nanostructures in Specific Proteasome Activation for Cancer Cell Pyroptosis

Authors - Qian-Wei Luo
mouseWB

Nat Commun

BNC1 deficiency-triggered ferroptosis through the NF2-YAP pathway induces primary ovarian insufficiency

Authors - Feixia Wang
ratWB

Acta Biomater

Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury.

Authors - Shanshan Ma
humanWB

Biomaterials

Autophagy-inhibiting polymer as an effective nonviral cancer gene therapy vector with inherent apoptosis-sensitizing ability.

Authors - Jiafeng Wang
humanWB

ACS Appl Mater Interfaces

Endoplasmic Reticulum-Targeted Aggregation-Induced Emission Luminogen for Synergetic Tumor Ablation with Glibenclamide

Authors - Yifan Wu