验证数据展示
经过测试的应用
| Positive WB detected in | SGC-7901 cells, HepG2 cells, THP-1 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11856-1-AP targets Caspase 4/P20/P10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag2429 Product name: Recombinant human Caspase 4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-377 aa of BC017839 Sequence: MAEGNHRKKPLKVLESLGKDFLTGVLDNLVEQNVLNWKEEEKKKYYDAKTEDKVRVMADSMQEKQRMAGQMLLQTFFNIDQISPNKKAHPNMEAGPPESGESTDALKLCPHEEFLRLCKERAEEIYPIKERNNRTRLALIICNTEFDHLPPRNGADFDITGMKELLEGLDYSVDVEENLTARDMESALRAFATRPEHKSSDSTFLVLMSHGILEGICGTVHDEKKPDVLLYDTIFQIFNNRNCLSLKDKPKVIIVQACRGANRGELWVRDSPASLEVASSQSSENLEEDAVYKTHVEKDFIAFCSSTPHNVSWRDSTMGSIFITQLITCFQKYSWCCHLEEVFRKVQQSFETPRAKAQMPTIERLSMTRYFYLFPGN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | caspase 4, apoptosis-related cysteine peptidase |
| 别名 | CASP4, Caspase4, CASP 4, CASP-4, Caspase 4 |
| 计算分子量 | 377 aa, 43 kDa |
| 观测分子量 | 43-48 kDa |
| GenBank蛋白编号 | BC017839 |
| 基因名称 | Caspase 4 |
| Gene ID (NCBI) | 837 |
| RRID | AB_2070053 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P49662 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Caspase 4/P20/P10 antibody 11856-1-AP | Download protocol |
| IHC protocol for Caspase 4/P20/P10 antibody 11856-1-AP | Download protocol |
| WB protocol for Caspase 4/P20/P10 antibody 11856-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Theranostics Outer membrane vesicles derived from gut microbiota mediate tubulointerstitial inflammation: a potential new mechanism for diabetic kidney disease
| ||
Phytomedicine Regulation of NLRP3 inflammasome and Caspase-3/4/11 by 2',4'-dihydroxychalcone contributes to anti-colorectal cancer | ||
Microbiol Res Caspase-4/11-mediated non-canonical pyroptosis disrupts blood-brain barrier integrity via VEGFA/VEGFR2 signaling in Escherichia coli meningitis. | ||
Front Immunol Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma
| ||
J Ethnopharmacol Aurantii Fructus alleviates depression via modulating hippocampal caspase-4-linked neurogenesis and synaptic plasticity in mice. | ||
Front Microbiol Enterovirus 71 Induces INF2 Cleavage via Activated Caspase-2 in Infected RD Cells. |
