验证数据展示

WB analysis using 10380-1-AP
Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 10380-1-AP (Caspase 9/P35/P10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10380-1-AP
IP result of anti-Caspase 9/P35/P10 (IP:10380-1-AP, 3ug; Detection:10380-1-AP 1:200) with HeLa cells lysate 2500 ug.
IHC staining of human heart using 10380-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10380-1-AP (Caspase 9/P35/P10 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 10380-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10380-1-AP (Caspase 9/P35/P10 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse lung using 10380-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10380-1-AP (Caspase 9/P35/P10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 10380-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10380-1-AP (Caspase 9/P35/P10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 10380-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Caspase 9/P35/P10 antibody (10380-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Featured Product
KD/KO验证

Caspase 9/P35/P10 Polyclonal antibody

Caspase 9/P35/P10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10380-1-AP

产品说明书

引用文献(808)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (8)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

别名

CASP9, Caspase 9, APAF 3, APAF3, APAF-3

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®555

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (808)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	Jurkat cells, Staurosporine treated Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse lung tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:300-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 772 publications below
IHC	See 38 publications below
IF	See 15 publications below
ELISA	See 1 publications below
RIP	See 1 publications below

产品信息

10380-1-AP targets Caspase 9/P35/P10 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, RIP, ELISA
经测试反应性	human, mouse
文献引用反应性	human, mouse, rat, pig, rabbit, chicken, zebrafish, hamster, sheep, goat
免疫原	Caspase 9/P35/P10 fusion protein Ag0404 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	caspase 9, apoptosis-related cysteine peptidase
别名	CASP9, Caspase 9, APAF 3, APAF3, APAF-3
计算分子量	46 kDa
观测分子量	46 kDa, 30-39 kDa
GenBank蛋白编号	BC002452
基因名称	Caspase 9
Gene ID (NCBI)	842
RRID	AB_2068632
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P55211
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Caspase 9, apoptosis-related cysteine protease (CASP9, synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Caspase 9 is processed by APAF1; this step is thought to be one of the earliest in the caspase activation cascade. 10380-1-AP can recognize the pre- and cleaved- caspase 9. In recent years, the localization of caspase 9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase 9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731].

实验方案

Product Specific Protocols
WB protocol for Caspase 9/P35/P10 antibody 10380-1-AP	Download protocol
IHC protocol for Caspase 9/P35/P10 antibody 10380-1-AP	Download protocol
IF protocol for Caspase 9/P35/P10 antibody 10380-1-AP	Download protocol
IP protocol for Caspase 9/P35/P10 antibody 10380-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Mol Cancer hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma Authors - Lei Xu View Article
mouse	WB,IHC	Bioact Mater Silicate ions as soluble form of bioactive ceramics alleviate aortic aneurysm and dissection Authors - Yumei Que View Article
rat	WB	Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model Authors - Mengyan Hu View Article
human	WB	Acta Pharm Sin B Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis. Authors - Xiaowei Zhang View Article
human	WB	Carbohydr Polym A multi-bioresponsive self-assembled nano drug delivery system based on hyaluronic acid and geraniol against liver cancer Authors - Shaofeng Duan View Article
rat	WB	Adv Healthc Mater A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction. Authors - Zhi Zheng View Article

验证数据展示

WB analysis using 10380-1-AP

IP experiment of HeLa using 10380-1-AP

IHC staining of human heart using 10380-1-AP

IHC staining of human heart using 10380-1-AP

IHC staining of mouse lung using 10380-1-AP

IHC staining of mouse lung using 10380-1-AP

IF Staining of HepG2 using 10380-1-AP

Caspase 9/P35/P10 Polyclonal antibody

Caspase 9/P35/P10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma

Silicate ions as soluble form of bioactive ceramics alleviate aortic aneurysm and dissection

Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model

Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis.

A multi-bioresponsive self-assembled nano drug delivery system based on hyaluronic acid and geraniol against liver cancer

A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.