CD107a / LAMP1 Polyclonal antibody

CD107a / LAMP1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 21997-1-AP

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Synonyms

CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker



经过测试的应用

Positive WB detected inHepG2 cells, U937 cells
Positive IHC detected inhuman liver cancer tissue, human placenta tissue, human gliomas tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy LAMP1/CD107a Ready-To-Use IHC Kit. LAMP1/CD107a primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21997-1-AP targets CD107a / LAMP1 in WB, IP, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IP, FC, IHC
Tested Reactivity human
Cited Reactivityhuman, turtle, monkey, zebrafish, pig, bovine
Immunogen CD107a / LAMP1 fusion protein Ag17367 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lysosomal-associated membrane protein 1
Synonyms CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker
Calculated Molecular Weight 417 aa, 45 kDa
Observed Molecular Weight 90-120 kDa
GenBank Accession NumberBC021288
Gene Symbol LAMP1
Gene ID (NCBI) 3916
ENSEMBL Gene IDENSG00000185896
RRIDAB_2878966
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11279
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.

实验方案

Product Specific Protocols
WB protocol for CD107a / LAMP1 antibody 21997-1-APDownload protocol
IHC protocol for CD107a / LAMP1 antibody 21997-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Immunity

The Endotoxin Delivery Protein HMGB1 Mediates Caspase-11-Dependent Lethality in Sepsis.

Authors - Meihong Deng
human

Adv Sci (Weinh)

Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy

Authors - Qian Gou
WB

Arthritis Rheumatol

LAMP3 induces lysosome-dependent cell death by impairing autophagic caspase-8 degradation in Sjögren's salivary glands

Authors - Hiroyuki Nakamura
human

Mol Ther

Polyphyllin D "Punctures" Hypertrophic Lysosomes to Reverse Drug Resistance of Hepatocellular Carcinoma by Targeting Acid Sphingomyelinase

Authors - Yang Wang
human

Nat Commun

Mismatch repair deficiency predicts response to HER2 blockade in HER2-negative breast cancer.

Authors - Nindo B Punturi
humanWB

Sci Total Environ

TFEB coordinates autophagy and pyroptosis as hepatotoxicity responses to ZnO nanoparticles

Authors - Xingyao Pei