CoraLite®594 Anti-Human CD107b / LAMP2 (H4B4)
CD107b / LAMP2 Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Human
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
H4B4
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | Jurkat cells |
Positive FC detected in | Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy LAMP2 Ready-To-Use IHC Kit. LAMP2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65053-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-65053 targets CD107b / LAMP2 in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | Human |
Immunogen | Human adherent peripheral blood cells 种属同源性预测 |
Host / Isotype | Mouse / IgG1, kappa |
Class | Monoclonal |
Type | Antibody |
Full Name | lysosomal-associated membrane protein 2 |
Synonyms | CD107b, Cell and organelle markers, LAMP 2, LAMP2, LAMPB, LGP110, Lysosome Marker |
Calculated Molecular Weight | 45 kDa |
GenBank Accession Number | BC002965 |
Gene Symbol | LAMP2 |
Gene ID (NCBI) | 3920 |
ENSEMBL Gene ID | ENSG00000005893 |
RRID | AB_2883469 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P13473 |
Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).
实验方案
Product Specific Protocols | |
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IF protocol for CL594 CD107b / LAMP2 antibody CL594-65053 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |