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验证数据展示

  • FC experiment of Jurkat using CL647-65053

    FC experiment of Jurkat using CL647-65053

    1X10^6 Jurkat cells were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD107b (CL647-65053, Clone:H4B4) (red) or Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL647-65053

    IF Staining of HeLa using CL647-65053

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 647-conjugated CD107b antibody (CL647-65053, Clone: H4B4) at dilution of 1:50 and CoraLite®488-conjugated CD107a antibody (CL488-65051, Clone: H4A3, green) at dilution of 1:50.

CoraLite® Plus 647 Anti-Human CD107b / LAMP2 (H4B4)

CD107b / LAMP2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-65053

产品说明书

CloneNo. H4B4

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human

应用

IF/ICC, FC (Intra)

别名

CD107b, Cell and organelle markers, LAMP 2, LAMP2, LAMPB, LGP110, Lysosome Marker

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy LAMP2 Ready-To-Use IHC Kit. LAMP2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65053-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65053 targets CD107b / LAMP2 in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 Human
免疫原 Human adherent peripheral blood cells 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 lysosomal-associated membrane protein 2
别名 CD107b, Cell and organelle markers, LAMP 2, LAMP2, LAMPB, LGP110, Lysosome Marker
计算分子量 45 kDa
GenBank蛋白编号BC002965
基因名称 LAMP2
Gene ID (NCBI) 3920
ENSEMBL Gene IDENSG00000005893
RRIDAB_2883656
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP13473
储存缓冲液 PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 CD107b / LAMP2 antibody CL647-65053Download protocol
Standard Protocols
Click here to view our Standard Protocols