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CD11B/Integrin Alpha M Monoclonal antibody, PBS Only

CD11B/Integrin Alpha M Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C7C2

Cat No : 66519-1-PBS

Print datasheet

Synonyms

CD11B, CD11B/Integrin Alpha M, CR 3 alpha chain, CR3A, Integrin alpha M, ITGAM, MAC 1, MAC1A, MO1A, Neutrophil adherence receptor, SLEB6

验证数据展示

  • WB analysis of THP-1 using 66519-1-Ig (same clone as 66519-1-PBS)

    WB analysis of THP-1 using 66519-1-Ig (same clone as 66519-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66519-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66519-1-Ig (same clone as 66519-1-PBS)

    IHC staining of human tonsillitis using 66519-1-Ig (same clone as 66519-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66519-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD11B antibody included
  • IHC staining of human tonsillitis using 66519-1-Ig (same clone as 66519-1-PBS)

    IHC staining of human tonsillitis using 66519-1-Ig (same clone as 66519-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66519-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD11B antibody included
  • IF Staining of human tonsillitis using 66519-1-Ig (same clone as 66519-1-PBS)

    IF Staining of human tonsillitis using 66519-1-Ig (same clone as 66519-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66519-1-Ig (CD11B/Integrin alpha M antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66519-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CD11B Ready-To-Use IHC Kit. CD11B primary antibody included.

产品信息

66519-1-PBS targets CD11B/Integrin Alpha M in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Synonyms CD11B, CD11B/Integrin Alpha M, CR 3 alpha chain, CR3A, Integrin alpha M, ITGAM, MAC 1, MAC1A, MO1A, Neutrophil adherence receptor, SLEB6
Calculated Molecular Weight 93 kDa
Observed Molecular Weight 160 kDa
GenBank Accession NumberNM_000632
Gene Symbol ITGAM
Gene ID (NCBI) 3684
ENSEMBL Gene IDENSG00000169896
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11215
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).