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CoraLite® Plus 488 Anti-Human CD11b (ICRF44) Mouse IgG2a Recombinant Antibody

CD11b Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

ICRF44

Cat No : CL488-65582

Print datasheet

Synonyms

ICRF44, CR3A, CR-3 alpha chain, CR 3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha

验证数据展示

  • FC experiment of human PBMCs using CL488-65582

    FC experiment of human PBMCs using CL488-65582

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD14 and 5 ul CoraLite® Plus 488 Anti-Human CD11b Mouse IgG2a Recombinant Antibody (CL488-65582, Clone: ICRF44), or CoraLite® Plus 488 Mouse IgG2a Isotype Control (CL488-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using CL488-65582

    FC experiment of human PBMCs using CL488-65582

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD11b Mouse IgG2a Recombinant Antibody (CL488-65582, Clone: ICRF44) (red), or CoraLite® Plus 488 Mouse IgG2a Isotype Control (CL488-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD11B Ready-To-Use IHC Kit. CD11B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65582-1-MR

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65582 targets CD11b in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Rheumatoid synovial cells and human monocytes 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Synonyms ICRF44, CR3A, CR-3 alpha chain, CR 3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha
Calculated Molecular Weight 1152 aa, 127 kDa
GenBank Accession NumberBC096346
Gene Symbol CD11b
Gene ID (NCBI) 3684
ENSEMBL Gene IDENSG00000169896
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CD11b antibody CL488-65582Download protocol
Standard Protocols
Click here to view our Standard Protocols