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验证数据展示

  • FC experiment of human PBMCs using CL594-65582

    FC experiment of human PBMCs using CL594-65582

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD14, and 5 ul CoraLite®594 Anti-Human CD11b (ICRF44) Mouse IgG2a Recombinant Antibody (CL594-65582, Clone: ICRF44) (right), or CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (left). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using CL594-65582

    FC experiment of human PBMCs using CL594-65582

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD11b (ICRF44) Mouse IgG2a Recombinant Antibody (CL594-65582, Clone: ICRF44)(red), or CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CoraLite®594 Anti-Human CD11b (ICRF44) Mouse IgG2a Recombinant Antibody

CD11b Uni-rAbTM Recombinant Antibody for FC
Cat No. CL594-65582

产品说明书

CloneNo. ICRF44

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC

别名

ICRF44, CR3A, CR-3 alpha chain, CR 3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD11B Ready-To-Use IHC Kit. CD11B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65582-1-MR

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65582 targets CD11b in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Rheumatoid synovial cells and human monocytes 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 integrin, alpha M (complement component 3 receptor 3 subunit)
别名 ICRF44, CR3A, CR-3 alpha chain, CR 3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha
计算分子量 1152 aa, 127 kDa
GenBank蛋白编号BC096346
基因名称 CD11b
Gene ID (NCBI) 3684
ENSEMBL Gene IDENSG00000169896
RRIDAB_3673489
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

实验方案

Product Specific Protocols
FC protocol for CL594 CD11b antibody CL594-65582Download protocol
Standard Protocols
Click here to view our Standard Protocols