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验证数据展示

  • WB analysis using 66443-1-Ig (same clone as 66443-1-PBS)

    WB analysis using 66443-1-Ig (same clone as 66443-1-PBS)

    Untreated and PNGase F-treated lysates of TF-1 cells, MOLT-4 cells, Jurkat cells were subjected to SDS PAGE followed by western blot with 66443-1-Ig (CD147 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 66443-1-Ig (same clone as 66443-1-PBS)

    IF Staining of A431 using 66443-1-Ig (same clone as 66443-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD147 antibody (66443-1-Ig, Clone: 1G12B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66443-1-Ig (same clone as 66443-1-PBS)

    IF Staining of HeLa using 66443-1-Ig (same clone as 66443-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD147 antibody (66443-1-Ig, Clone: 1G12B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66443-1-Ig (same clone as 66443-1-PBS)

    IHC staining of human liver cancer using 66443-1-Ig (same clone as 66443-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66443-1-Ig (CD147 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD147/BSG antibody included
  • IHC staining of human liver cancer using 66443-1-Ig (same clone as 66443-1-PBS)

    IHC staining of human liver cancer using 66443-1-Ig (same clone as 66443-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66443-1-Ig (CD147 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD147/BSG antibody included
  • IF Staining of human tonsillitis using 66443-1-Ig (same clone as 66443-1-PBS)

    IF Staining of human tonsillitis using 66443-1-Ig (same clone as 66443-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD147 antibody (66443-1-Ig, Clone: 1G12B5 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66443-1-Ig (same clone as 66443-1-PBS)

    IF Staining of human tonsillitis using 66443-1-Ig (same clone as 66443-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD147 antibody (66443-1-Ig, Clone: 1G12B5 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50494-1

    Cytometric bead array standard curve of MP50494-1

    Cytometric bead array standard curve of MP50494-1, CD147 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66443-2-PBS. Detection antibody: 66443-1-PBS. Standard:Ag2619. Range: 0.098-3.125 ng/mL.

  • Featured Product
  • KD/KO验证

CD147 Monoclonal antibody, PBS Only (Detector)

CD147 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 66443-1-PBS

产品说明书

CloneNo. 1G12B5

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

别名

EMMPRIN, Collagenase stimulatory factor, BSG, Basigin, 5F7

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD147/BSG Ready-To-Use IHC Kit. CD147/BSG primary antibody included.

产品信息

66443-1-PBS targets CD147 as part of a matched antibody pair:

MP50494-1: 66443-2-PBS capture and 66443-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原 CD147 fusion protein Ag2619 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 basigin (Ok blood group)
别名 EMMPRIN, Collagenase stimulatory factor, BSG, Basigin, 5F7
计算分子量 385 aa, 42 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC009040
基因名称 CD147
Gene ID (NCBI) 682
RRIDAB_2881813
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35613
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020). The CD147 gene, designated BSG for basigin, is located on chromosome 19p13.3. Four transcript variants encoding different isoforms have been found (PMID: 21536654). CD147 migrates on SDS-PAGE usually between 35 and 65 kDa, depending on the degree of glycosylation (PMID: 17945211).