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验证数据展示

  • FC experiment of human PBMCs using 65090-1-Ig (same clone as 65090-1-PBS)

    FC experiment of human PBMCs using 65090-1-Ig (same clone as 65090-1-PBS)

    1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD3 and 0.2 ug Anti-Human CD16 (65090-1-Ig, Clone:3G8) and APC-Goat anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65090-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65090-1-Ig (same clone as 65090-1-PBS)

    FC experiment of human PBMCs using 65090-1-Ig (same clone as 65090-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD16 (65090-1-Ig, Clone:3G8) and APC-Goat anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65090-1-PBS in a different storage buffer formulation.

  • Sample test of MP50298-3

    Sample test of MP50298-3

    Cytometric bead array of Human urine using MP50298-3, CD16a Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 65090-1-PBS. Detection antibody: 68897-2-PBS. Urine of four individual healthy human donors was analyzed using MP50298-3.

  • Sample test of MP50298-4

    Sample test of MP50298-4

    Cytometric bead array of Human urine using MP50298-4, CD16a Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 65090-1-PBS. Detection antibody: 68897-3-PBS. Urine of four individual healthy human donors was analyzed using MP50298-4.

  • Cytometric bead array standard curve of MP50298-3

    Cytometric bead array standard curve of MP50298-3

    Cytometric bead array standard curve of MP50298-3, CD16 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 65090-1-PBS. Detection antibody: 68897-2-PBS. Standard:Eg31662. Range: 1.563-100 ng/mL.

  • Cytometric bead array standard curve of MP50298-4

    Cytometric bead array standard curve of MP50298-4

    Cytometric bead array standard curve of MP50298-4, CD16 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 65090-1-PBS. Detection antibody: 68897-3-PBS. Standard:Eg31662. Range: 0.781-100 ng/mL.

Anti-Human CD16 (3G8), PBS Only (Capture)

CD16 Monoclonal Antibody for FC, Cytometric bead array, Indirect ELISA, Sample test
Cat No. 65090-1-PBS

产品说明书

CloneNo. 3G8

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human

应用

FC, Cytometric bead array, Indirect ELISA, Sample test

别名

3G8, CD16A, CD16a antigen, CD16-II, Fc gamma RIII

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD16 Ready-To-Use IHC Kit. CD16 primary antibody included.

产品信息

65090-1-PBS targets CD16 as part of a matched antibody pair:

MP50298-3: 65090-1-PBS capture and 68897-2-PBS detection (validated in Cytometric bead array)

MP50298-4: 65090-1-PBS capture and 68897-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 FC, Cytometric bead array, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
别名 3G8, CD16A, CD16a antigen, CD16-II, Fc gamma RIII
计算分子量 254 aa, 29 kDa
GenBank蛋白编号BC017865
基因名称 CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
RRIDAB_2918393
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).