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CD19 Polyclonal antibody

CD19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27949-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

Differentiation antigen CD19, CD19 molecule, B-lymphocyte surface antigen B4, B-lymphocyte antigen CD19, B4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inDaudi cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue, mouse colon tissue, rat colon tissue, rat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27949-1-AP targets CD19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 CD19 fusion protein Ag27656 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD19 molecule
别名 Differentiation antigen CD19, CD19 molecule, B-lymphocyte surface antigen B4, B-lymphocyte antigen CD19, B4
计算分子量 556 aa, 61 kDa
观测分子量 95 kDa
GenBank蛋白编号BC006338
基因名称 CD19
Gene ID (NCBI) 930
ENSEMBL Gene IDENSG00000177455
RRIDAB_2881019
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15391
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD19 is a B cell-specific molecule that controls B cell activation by complexing with the BCR. CD19 is a member of the Ig superfamily and is the dominant component for the signaling complex on B cells that includes CD21, CD81, and CD22. Humans with CD19 deficiency have reduced proliferative responses to BCR stimulation in vitro and mount impaired Ab responses to vaccination.

实验方案

Product Specific Protocols
WB protocol for CD19 antibody 27949-1-APDownload protocol
IHC protocol for CD19 antibody 27949-1-APDownload protocol
IF protocol for CD19 antibody 27949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIHC

J Adv Res

Zearalenone-14-glucoside specifically promotes dysplasia of Gut-Associated Lymphoid Tissue: A natural product for constructing intestinal nodular lymphatic hyperplasia model

Authors - Haonan Ruan
humanIHC

Int J Mol Sci

CXCR4 Expressed by Tumor-Infiltrating B Cells in Gastric Cancer Related to Survival in the Tumor Microenvironment: An Analysis Combining Single-Cell RNA Sequencing with Bulk RNA Sequencing

Authors - Chen Su
humanWB

Int J Biol Macromol

Exosome co-delivery of a STING agonist augments immunogenicity elicited by CVB3 VP1 vaccine via promoting antigen cross-presentation of CD8 + DCs

Authors - Changwei Zhang
humanIF

bioRxiv

A Novel Humanized Mouse Model for HIV and Tuberculosis Co-infection Studies

Authors - José Alejandro Bohórquez
humanIHC

Cancer Lett

Comprehensive molecular characterization of long-term glioblastoma survivors

Authors - Hao Xu
IHC

J Ethnopharmacol

Effective substances and molecular mechanisms guided by network pharmacology: An example study of Scrophulariae Radix treatment of hyperthyroidism and thyroid hormone-induced liver and kidney injuries

Authors - Meng-Yuan Sheng
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