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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • FC experiment of C57BL/6 mouse splenocytes using 65290-1-Ig

    FC experiment of C57BL/6 mouse splenocytes using 65290-1-Ig

    1X10^6 C57BL/6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug Anti-Mouse CD19 (65290-1-Ig, Clone:1D3) or 0.5 ug Rat IgG2a Isotype Control (2A3), and FITC anti-rat IgG2a Antibody at dilution 1:100. Cells were not fixed.

  • IF Staining of mouse spleen using 65290-1-Ig

    IF Staining of mouse spleen using 65290-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using CD19 antibody (65290-1-Ig, Clone: 1D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Anti-Mouse CD19 (1D3)

CD19 Monoclonal Antibody for IF-Fro, FC
Cat No. 65290-1-Ig

产品说明书

CloneNo. 1D3

宿主/亚型

Rat / IgG2a, kappa

种属反应性

mouse

应用

IF-Fro, FC

别名

CD19 antigen, 1D3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IF-Fro detected inmouse spleen tissue
Positive FC detected inmouse splenocytes

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-FROIF-FRO : 1:200-1:800
Flow Cytometry (FC)FC : 0.5 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65290-1-Ig targets CD19 in IF-Fro, FC applications and shows reactivity with mouse samples.

经测试应用 IF-Fro, FC Application Description
经测试反应性 mouse
免疫原 Mouse CD19 Transfected Cell Line 种属同源性预测
宿主/亚型 Rat / IgG2a, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD19 antigen
别名 CD19 antigen, 1D3
GenBank蛋白编号BC156767
基因名称 Cd19
Gene ID (NCBI) 12478
RRIDAB_3084916
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

实验方案

Product Specific Protocols
IF protocol for CD19 antibody 65290-1-IgDownload protocol
FC protocol for CD19 antibody 65290-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols