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Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody, PBS Only

CD19 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

4G7

Cat no : 65562-1-PBS

Print datasheet

Synonyms

CD19 molecule, B-lymphocyte surface antigen B4, B-lymphocyte antigen CD19, B4, B lymphocyte antigen CD19

验证数据展示

  • FC experiment of human PBMCs using 65562-1-MR (same clone as 65562-1-PBS)

    FC experiment of human PBMCs using 65562-1-MR (same clone as 65562-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD19 Mouse IgG2a Recombinant Antibody (65562-1-MR, Clone: 4G7) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65562-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65562-1-MR (same clone as 65562-1-PBS)

    FC experiment of human PBMCs using 65562-1-MR (same clone as 65562-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD19 Mouse IgG2a Recombinant Antibody (65562-1-MR, Clone: 4G7) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65562-1-PBS in a different storage buffer formulation.

产品信息

65562-1-PBS targets CD19 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Human chronic lymphocytic leukemia (CLL) cells 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name CD19 molecule
Synonyms CD19 molecule, B-lymphocyte surface antigen B4, B-lymphocyte antigen CD19, B4, B lymphocyte antigen CD19
Calculated Molecular Weight 556 aa, 61 kDa
GenBank Accession NumberBC006338
Gene Symbol CD19
Gene ID (NCBI) 930
ENSEMBL Gene IDENSG00000177455
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP15391
Storage Buffer PBS Only
Storage ConditionsStore at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).