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验证数据展示

  • WB analysis using 60271-1-Ig (same clone as 60271-1-PBS)

    WB analysis using 60271-1-Ig (same clone as 60271-1-PBS)

    Raji cells (CD20+), Daudi cells (CD20+), Ramos cells (CD20+), Jurkat cells (CD20-), and HeLa cells (CD20-) were subjected to SDS PAGE followed by western blot with 60271-1-Ig (CD20 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    IHC staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD20 antibody included
  • IHC staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    IHC staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD20 antibody included
  • IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (60271-1-Ig, Clone: 4A7G3 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD3 antibody (17617-1-AP, green). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (60271-1-Ig, Clone: 4A7G3 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD3 antibody (17617-1-AP, green). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • IF Staining of Raji using 60271-1-Ig (same clone as 60271-1-PBS)

    IF Staining of Raji using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD20 antibody (60271-1-Ig, Clone: 4A7G3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    IHC staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD20 antibody included

CD20 Monoclonal antibody, PBS Only

CD20 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 60271-1-PBS

产品说明书

CloneNo. 4A7G3

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

Bp35, B-lymphocyte surface antigen B1, B-lymphocyte antigen CD20, B1, B lymphocyte antigen CD20

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD20 Ready-To-Use IHC Kit. CD20 primary antibody included.

产品信息

60271-1-PBS targets CD20 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原 CD20 fusion protein Ag17309 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 membrane-spanning 4-domains, subfamily A, member 1
别名 Bp35, B-lymphocyte surface antigen B1, B-lymphocyte antigen CD20, B1, B lymphocyte antigen CD20
计算分子量 297 aa, 33 kDa
观测分子量 35 kDa
GenBank蛋白编号BC002807
基因名称 CD20
Gene ID (NCBI) 931
RRIDAB_2881391
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP11836
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.