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验证数据展示

  • FC experiment of human PBMCs using 65575-1-MR (same clone as 65575-1-PBS)

    FC experiment of human PBMCs using 65575-1-MR (same clone as 65575-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD20 Mouse IgG2a Recombinant Antibody (65575-1-MR, Clone: 2H7) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65575-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65575-1-MR (same clone as 65575-1-PBS)

    FC experiment of human PBMCs using 65575-1-MR (same clone as 65575-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD20 Mouse IgG2a Recombinant Antibody (65575-1-MR, Clone: 2H7) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65575-1-PBS in a different storage buffer formulation.

  • FC experiment of Cnyo PBMCs using 65575-1-MR (same clone as 65575-1-PBS)

    FC experiment of Cnyo PBMCs using 65575-1-MR (same clone as 65575-1-PBS)

    1x10^6 cynomolgus PBMCs were surface stained with 0.25 ug Anti-Human CD20 (2H7) Mouse IgG2a RecAb (65575-1-MR, Clone: 2H7) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. This data was developed using the same antibody clone with 65575-1-PBS in a different storage buffer formulation.

  • FC experiment of Cnyo PBMCs using 65575-1-MR (same clone as 65575-1-PBS)

    FC experiment of Cnyo PBMCs using 65575-1-MR (same clone as 65575-1-PBS)

    1x10^6 cynomolgus PBMCs were surface stained with 0.25 ug Anti-Human CD20 (2H7) Mouse IgG2a RecAb (65575-1-MR, Clone: 2H7) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. This data was developed using the same antibody clone with 65575-1-PBS in a different storage buffer formulation.

Anti-Human CD20 (2H7) Mouse IgG2a Recombinant Antibody, PBS Only

CD20 Uni-rAb® Recombinant Antibody for FC
Cat No. 65575-1-PBS

产品说明书

CloneNo. 2H7

宿主/亚型

Mouse / IgG2a

种属反应性

human, monkey, non-human primates

应用

FC

别名

2H7, B lymphocyte antigen CD20, B1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CD20 Ready-To-Use IHC Kit. CD20 primary antibody included.

产品信息

65575-1-PBS targets CD20 in FC applications and shows reactivity with human, monkey, non-human primates samples.

经测试应用 FC Application Description
经测试反应性 human, monkey, non-human primates
免疫原

Human tonsillar B cells

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 membrane-spanning 4-domains, subfamily A, member 1
别名 2H7, B lymphocyte antigen CD20, B1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1
计算分子量 297 aa, 33 kDa
GenBank蛋白编号BC002807
基因名称 CD20
Gene ID (NCBI) 931
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP11836-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).

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