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  • Featured Product
  • KD/KO Validated

CD209 Polyclonal antibody

CD209 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25404-1-AP

Print datasheet

Synonyms

CD209, CD209 antigen, CD209 molecule, CDSIGN, CLEC4L, DC SIGN, DC SIGN1

验证数据展示

  • WB analysis of human placenta using 25404-1-AP

    WB analysis of human placenta using 25404-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 25404-1-AP (CD209 Antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • FC experiment of U-937 using 25404-1-AP

    FC experiment of U-937 using 25404-1-AP

    1X10^6 U-937 cells were stained with 0.2ug CD209 antibody (25404-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive FC detected inU-937 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25404-1-AP is CD209 Fusion Protein expressed in E. coli.

Tested Applications FC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen CD209 fusion protein Ag21863 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD209 molecule
Synonyms CD209, CD209 antigen, CD209 molecule, CDSIGN, CLEC4L, DC SIGN, DC SIGN1
Calculated Molecular Weight 404 aa, 45.7 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC110615
Gene Symbol CD209
Gene ID (NCBI) 30835
RRIDAB_2880062
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NNX6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD209 (DC-SIGN, CLEC4L) is a C-type lectin receptor that is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens, including bacteria, viruses, and parasites. CD209 is preferentially expressed on dendritic cells (DCs). It mediates transient adhesion of DCs with T cells. It has been reported that CD209 plays a role in capture and transmission of HIV from DC to T cells (PMID: 10721995).

实验方案

Product Specific Protocols
WB protocol for CD209 antibody 25404-1-APDownload protocol
FC protocol for CD209 antibody 25404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Immunity

SARS-CoV-2 exacerbates proinflammatory responses in myeloid cells through C-type lectin receptors and Tweety family member 2.

Authors - Qiao Lu
  • KD Validated
View Article