Anti-Human CD3 (OKT3)

CD3 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

OKT3

Cat No : 65133-1-Ig

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Synonyms

CD3, CD3E, T3E, TCRE



经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD3 Ready-To-Use IHC Kit. CD3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

产品信息

65133-1-Ig targets CD3 in FC applications and shows reactivity with Human samples.

Tested Applications FC
Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Synonyms CD3, CD3E, T3E, TCRE
Calculated molecular weight 207 aa, 23 kDa
GenBank accession numberBC049847
Gene symbol CD3E
UniprotP07766
Gene ID (NCBI) 916
RRIDAB_2918421
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide, pH 7.2.
Storage ConditionsStore at 2-8°C.

背景介绍

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

实验方案

Product Specific Protocols
FC protocol for CD3 antibody 65133-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols