• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CD31 Monoclonal antibody, PBS Only (Capture)

CD31 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F8E2

Cat no : 66065-2-PBS

Print datasheet

Synonyms

PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM

验证数据展示

  • WB analysis using 66065-2-Ig (same clone as 66065-2-PBS)

    WB analysis using 66065-2-Ig (same clone as 66065-2-PBS)

    Untreated and PNGase F-treated lysates of human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • IF Staining of HUVEC using 66065-2-Ig (same clone as 66065-2-PBS)

    IF Staining of HUVEC using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • WB analysis of human peripheral blood platelets using 66065-2-Ig (same clone as 66065-2-PBS)

    WB analysis of human peripheral blood platelets using 66065-2-Ig (same clone as 66065-2-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66065-2-Ig (same clone as 66065-2-PBS)

    IHC staining of human liver cancer using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human liver cancer using 66065-2-Ig (same clone as 66065-2-PBS)

    IHC staining of human liver cancer using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD31 antibody included
  • WB analysis using 66065-2-Ig (same clone as 66065-2-PBS)

    WB analysis using 66065-2-Ig (same clone as 66065-2-PBS)

    Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • IF Staining of human placenta using 66065-2-Ig (same clone as 66065-2-PBS)

    IF Staining of human placenta using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • IF Staining of human placenta using 66065-2-Ig (same clone as 66065-2-PBS)

    IF Staining of human placenta using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • IF Staining of HUVEC using 66065-2-Ig (same clone as 66065-2-PBS)

    IF Staining of HUVEC using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), Beta Tubulin antibody (80713-1-RR, Clone: 2O13, red). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50319-2

    Cytometric bead array standard curve of MP50319-2

    Cytometric bead array standard curve of MP50319-2, CD31 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66065-2-PBS. Detection antibody: 66065-5-PBS. Standard:Ag1787. Range: 3.125-200 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy CD31 Ready-To-Use IHC Kit. CD31 primary antibody included.

产品信息

66065-2-PBS targets CD31 as part of a matched antibody pair:

MP50319-2: 66065-2-PBS capture and 66065-5-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen CD31 fusion protein Ag19730 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name platelet/endothelial cell adhesion molecule
Synonyms PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM
Calculated Molecular Weight 83 kDa
Observed Molecular Weight120-130 kDa
GenBank Accession NumberBC022512
Gene Symbol CD31
Gene ID (NCBI) 5175
ENSEMBL Gene IDENSG00000261371
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP16284
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).