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CD31 Monoclonal antibody, PBS Only

CD31 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3F8E2

Cat No : 66065-2-PBS

Print datasheet

Synonyms

CD31, EndoCAM, FLJ58394, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker

验证数据展示

  • WB analysis using 66065-2-Ig (same clone as 66065-2-PBS)

    WB analysis using 66065-2-Ig (same clone as 66065-2-PBS)

    Untreated and PNGase F-treated lysates of human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • WB analysis of human peripheral blood platelets using 66065-2-Ig (same clone as 66065-2-PBS)

    WB analysis of human peripheral blood platelets using 66065-2-Ig (same clone as 66065-2-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66065-2-Ig (same clone as 66065-2-PBS)

    IHC staining of human liver cancer using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD31 antibody included
  • IHC staining of human liver cancer using 66065-2-Ig (same clone as 66065-2-PBS)

    IHC staining of human liver cancer using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD31 antibody included
  • WB analysis using 66065-2-Ig (same clone as 66065-2-PBS)

    WB analysis using 66065-2-Ig (same clone as 66065-2-PBS)

    Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • IF Staining of human placenta using 66065-2-Ig (same clone as 66065-2-PBS)

    IF Staining of human placenta using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • IF Staining of human placenta using 66065-2-Ig (same clone as 66065-2-PBS)

    IF Staining of human placenta using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CD31 Ready-To-Use IHC Kit. CD31 primary antibody included.

产品信息

66065-2-PBS targets CD31 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen CD31 fusion protein Ag19730 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name platelet/endothelial cell adhesion molecule
Synonyms CD31, EndoCAM, FLJ58394, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker
Calculated Molecular Weight 83 kDa
Observed Molecular Weight120-130 kDa
GenBank Accession NumberBC022512
Gene Symbol PECAM1
Gene ID (NCBI) 5175
ENSEMBL Gene IDENSG00000261371
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP16284
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).