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验证数据展示

  • WB analysis of human placenta using 60180-2-Ig

    WB analysis of human placenta using 60180-2-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 60180-2-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 60180-2-Ig

    IHC staining of human tonsillitis using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD34 antibody included
  • IHC staining of human tonsillitis using 60180-2-Ig

    IHC staining of human tonsillitis using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD34 antibody included
  • IHC staining of human appendicitis using 60180-2-Ig

    IHC staining of human appendicitis using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human pancreas using 60180-2-Ig

    IHC staining of human pancreas using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human tonsillitis using 60180-2-Ig

    IHC staining of human tonsillitis using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human ovary cancer using 60180-2-Ig

    IHC staining of human ovary cancer using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human pancreas cancer using 60180-2-Ig

    IHC staining of human pancreas cancer using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human colorectal cancer using 60180-2-Ig

    IHC staining of human colorectal cancer using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human liver cancer using 60180-2-Ig

    IHC staining of human liver cancer using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human placenta using 60180-2-Ig

    IHC staining of human placenta using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human placenta using 60180-2-Ig

    IHC staining of human placenta using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD34 antibody included
  • IHC staining of human liver cancer using 60180-2-Ig

    IHC staining of human liver cancer using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD34 antibody included
  • IHC staining of human colorectal cancer using 60180-2-Ig

    IHC staining of human colorectal cancer using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD34 antibody included
  • IHC staining of human pancreas cancer using 60180-2-Ig

    IHC staining of human pancreas cancer using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD34 antibody included
  • IHC staining of human ovary cancer using 60180-2-Ig

    IHC staining of human ovary cancer using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD34 antibody included
  • IHC staining of human tonsillitis using 60180-2-Ig

    IHC staining of human tonsillitis using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD34 antibody included
  • IHC staining of human pancreas using 60180-2-Ig

    IHC staining of human pancreas using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD34 antibody included
  • IHC staining of human liver using 60180-2-Ig

    IHC staining of human liver using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD34 antibody included
  • IHC staining of human appendicitis using 60180-2-Ig

    IHC staining of human appendicitis using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD34 antibody included
  • IHC staining of human tonsillitis using 60180-2-Ig

    IHC staining of human tonsillitis using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD34 antibody included
  • IHC staining of human breast cancer using 60180-2-Ig

    IHC staining of human breast cancer using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD34 antibody included
  • IHC staining of human liver cancer using 60180-2-Ig

    IHC staining of human liver cancer using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD34 antibody included
  • IHC staining of human tonsillitis using 60180-2-Ig

    IHC staining of human tonsillitis using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD34 antibody included
  • IHC staining of human liver using 60180-2-Ig

    IHC staining of human liver using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD34 antibody included
  • IHC staining of human tonsillitis using 60180-2-Ig

    IHC staining of human tonsillitis using 60180-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD34 antibody included

CD34 Monoclonal antibody

CD34 Monoclonal Antibody for WB, IHC, IHC-Autostainer, ELISA
Cat No. 60180-2-Ig

产品说明书

CloneNo. 4F11H3
引用文献(4)

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IHC-Autostainer, ELISA and More (1)

别名

4F11H3, CD34 molecule

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue, human ovary cancer cells, human uterus tissue, human testis tissue
Positive IHC-Autostainer detected inhuman colorectal cancer tissue, human placenta tissue, human liver cancer tissue, human pancreas cancer tissue, human ovary cancer tissue, human tonsillitis tissue, human pancreas tissue, human appendicitis tissue
Positive IHC detected inhuman tonsillitis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD34 Ready-To-Use IHC Kit. CD34 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)-AUTOSTAINERIHC-AUTOSTAINER : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60180-2-Ig targets CD34 in WB, IHC, IHC-Autostainer, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IHC-Autostainer, ELISA Application Description
文献引用应用IHC, IF
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag5996

Product name: Recombinant human CD34 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 34-287 aa of BC039146

Sequence: DNNGTATPELPTQGTFSNVSTNVSYQETTTPSTLGSTSLHPVSQHGNEATTNITETTVKFTSTSVITSVYGNTNSSVQSQTSVISTVFTTPANVSTPETTLKPSLSPGNVSDLSTTSTSLATSPTKPYTSSSPILSDIKAEIKCSGIREVKLTQGICLEQNKTSSCAEFKKDRGEGLARVLCGEEQADADAGAQVCSLLLAQSEVRPQCLLLVLANRTEISSKLQLMKKHQSDLKKLGILDFTEQDVASHQSYS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CD34 molecule
别名 4F11H3, CD34 molecule
计算分子量 41 kDa
观测分子量105 kDa
GenBank蛋白编号BC039146
基因名称 CD34
Gene ID (NCBI) 947
RRIDAB_2881354
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP28906
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. The calculated molecular mass of human CD34 is 41 kDa, various forms with different molecular weights may be produced due to different glycosylation patterns and alternative splicing (PMID: 24375067; 15750786).

实验方案

Product Specific Protocols
IHC protocol for CD34 antibody 60180-2-IgDownload protocol
WB protocol for CD34 antibody 60180-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Oncol

Prognostic Impact of the Angiogenic Gene POSTN and Its Related Genes on Lung Adenocarcinoma.

Authors - Dongfeng Sun
View Article

BMC Cancer

Novel fusion protein PK5-RL-Gal-3C inhibits hepatocellular carcinoma via anti-angiogenesis and cytotoxicity

Authors - Xiaoge Gao
View Article
humanIF

STAR Protoc

Skin-iDISCO+: An optimized tissue-clearing and labeling protocol for morphometric analysis of human cutaneous vasculature

Authors - Léa Pechtimaldjian
View Article
IF

Front Cell Neurosci

Decreased neuronal and increased endothelial fractalkine expression are associated with neuroinflammation in Parkinson's disease and related disorders

Authors - Yaping Chu
View Article
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