• Featured Product
  • KD/KO验证

CD36 Polyclonal antibody

CD36 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18836-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (3)

应用

WB, IHC, ELISA and More (1)

PAS 4, GPIV, GPIIIB, GP4, GP3B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHuman peripheral blood platelets
Positive IHC detected inhuman spleen tissue, human liver tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18836-1-AP targets CD36 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IP
经测试反应性 human
文献引用反应性human, pig, bovine, goat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD36 molecule (thrombospondin receptor)
别名 PAS 4, GPIV, GPIIIB, GP4, GP3B
计算分子量 472 aa, 53 kDa
观测分子量 88 kDa
GenBank蛋白编号BC008406
基因名称 CD36
Gene ID (NCBI) 948
RRIDAB_10597244
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16671
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Function

CD36, also known as platelet glycoprotein 4, is an integral membrane glycoprotein that acts as a scavenger receptor. CD36 can bind to multiple ligands, including thrombospondin-1, collagen, oxidized phospholipids, oxidized low-density lipoprotein, and long-chain fatty acids. CD36 can also bind to erythrocytes parasitized by Plasmodium falciparum and apoptotic cells. CD36 mediates different biological processes, acting as a signaling hub in angiogenesis, inflammatory response, and fatty acid metabolism.

Tissue specificity

CD36 is present on the surface of various cells types, such as adipocytes, monocytes, macrophages, platelets, microvascular endothelial cells, dendritic cells, and hematopoietic precursors of red cells.

Involvement in disease
  • Mutations in CD36 can give rise to platelet glycoprotein IV deficiency, a type of macrothrombocytopenia.

  • Polymorphisms in CD36 can increase susceptibility to malaria.

  • AAGIC haplotype at the CD36 locus increases the risk of coronary heart disease.

  • Disruption of CD36-dependent pathways and certain SNPs in the CD36 gene are attributed to impaired fatty acid metabolism, glucose intolerance, Alzheimer's disease, atherosclerosis, arterial hypertension, diabetes, and cardiomyopathy.

Isoforms

Apart from the full-length protein (isoform 1), one additional shorter isoform has been reported (PMID: 7509795). Other alternative isoforms have also been detected on the mRNA level (PMID: 17673938).

Post-translational modifications

The extracellular domain of CD36 is extensively glycosylated. Glycosylation is needed for the transport of CD36 to the plasma membrane, as well as mediating recognition and binding to ligands. Cytoplasmic tails of transmembrane domains can be phosphorylated and play a role in signal transduction. Intracellular domains can be additionally acetylated, ubiquitinated, and palmitoylated (PMID: 28919632).

Cellular localization

CD36 is present on the cell surface.            

实验方案

Product Specific Protocols
WB protocol for CD36 antibody 18836-1-APDownload protocol
IHC protocol for CD36 antibody 18836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Nat Commun

Suppression of angiopoietin-like 4 reprograms endothelial cell metabolism and inhibits angiogenesis

Authors - Balkrishna Chaube
humanIHC

Cell Rep Med

Targeting neoadjuvant chemotherapy-induced metabolic reprogramming in pancreatic cancer promotes anti-tumor immunity and chemo-response

Authors - Rong Tang
WB

Nat Commun

Deficiency of intestinal Bmal1 prevents obesity induced by high-fat feeding.

Authors - Fangjun Yu
WB

Nat Commun

The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression.

Authors - Xinzhi Li
humanWB

Nat Commun

Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise.

Authors - Jin-Ho Koh
WB

Nat Commun

Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH)

Authors - Xinzhi Li
Loading...