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Anti-Human CD4 (RPA-T4)

CD4 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human and More (1)

Applications

FC and More (1)

Conjugate

Unconjugated

CloneNo.

RPA-T4

Cat no : 65143-1-Ig

Print datasheet

Synonyms

CD4, CD4 molecule, CD4mut

验证数据展示

  • FC experiment of human PBMCs using 65143-1-Ig

    FC experiment of human PBMCs using 65143-1-Ig

    1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) or Mouse IgG1 Isotype Control and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD3 (CL488-65151). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65143-1-Ig

    FC experiment of human PBMCs using 65143-1-Ig

    1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) or Mouse IgG1 Isotype Control and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

65143-1-Ig targets CD4 in IF, FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Cited ApplicationsIF
Tested Reactivity Human
Cited Reactivityhuman
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD4 molecule
Synonyms CD4, CD4 molecule, CD4mut
Calculated Molecular Weight 55 kDa
GenBank Accession NumberBC025782
Gene Symbol CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
RRIDAB_2918429
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP01730
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

实验方案

Product Specific Protocols
FC protocol for CD4 antibody 65143-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Allergy Asthma Immunol Res

TET2 Regulates 5-Hydroxymethylcytosine Signature and CD4+ T-Cell Balance in Allergic Rhinitis.

Authors - Lu Tan
View Article