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Anti-Human CD45 Rabbit Recombinant Antibody

CD45 Uni-rAbTM Recombinant Antibody for IF/ICC, FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC

Conjugate

Unconjugated

CloneNo.

241670E10

Cat no : 98117-1-RR

Print datasheet

Synonyms

LCA, L CA, GP180, EC:3.1.3.48, B220

验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98117-1-RR

    FC experiment of human peripheral blood leukocytes using 98117-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD45 Rabbit Recombinant Antibody (98117-1-RR, Clone: 241670E10) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • IF Staining of Jurkat using 98117-1-RR

    IF Staining of Jurkat using 98117-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45 antibody (98117-1-RR, Clone: 241670E10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 98117-1-RR

    Affinity and Kinetic Characterization of 98117-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98117-1-RR against Human CD45 were performed. The affinity constant is 2.58 nM.

经过测试的应用

Positive IF/ICC detected inJurkat cells
Positive FC detected inhuman peripheral blood leukocytes
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98117-1-RR is CD45 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, FC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms LCA, L CA, GP180, EC:3.1.3.48, B220
Gene Symbol CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

实验方案

Product Specific Protocols
IF protocol for CD45 antibody 98117-1-RRDownload protocol
FC protocol for CD45 antibody 98117-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols