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验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98117-1-RR

    FC experiment of human peripheral blood leukocytes using 98117-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD45 Rabbit Recombinant Antibody (98117-1-RR, Clone: 241670E10) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • IF Staining of Jurkat using 98117-1-RR

    IF Staining of Jurkat using 98117-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45 antibody (98117-1-RR, Clone: 241670E10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 98117-1-RR

    Affinity and Kinetic Characterization of 98117-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98117-1-RR against Human CD45 were performed. The affinity constant is 2.58 nM.

Anti-Human CD45 Rabbit Recombinant Antibody

CD45 Uni-rAbTM Recombinant Antibody for IF/ICC, FC
Cat No. 98117-1-RR

产品说明书

CloneNo. 241670E10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC

别名

LCA, L CA, GP180, EC:3.1.3.48, B220

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inJurkat cells
Positive FC detected inhuman peripheral blood leukocytes
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98117-1-RR targets CD45 in IF/ICC, FC applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, C
别名 LCA, L CA, GP180, EC:3.1.3.48, B220
基因名称 CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_3672264
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

实验方案

Product Specific Protocols
IF protocol for CD45 antibody 98117-1-RRDownload protocol
FC protocol for CD45 antibody 98117-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols