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验证数据展示

  • FC experiment of human peripheral blood leukocytes using AY7-98117

    FC experiment of human peripheral blood leukocytes using AY7-98117

    1x10^6 human peripheral blood leukocytes were unstained or surface stained with 5 ul APC-Cyanine7 Anti-Human CD45 Rabbit Recombinant Antibody (AY7-98117, Clone: 241670E10). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using AY7-98117

    FC experiment of human peripheral blood leukocytes using AY7-98117

    1x10^6 human peripheral blood leukocytes were unstained (blue) or surface stained with 5 ul APC-Cyanine7 Anti-Human CD45 Rabbit Recombinant Antibody (AY7-98117, Clone: 241670E10) (red). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

APC-Cyanine7 Anti-Human CD45 Rabbit Recombinant Antibody

CD45 Recombinant Antibody for FC
Cat No. AY7-98117

Print datasheet

CloneNo. 241670E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

LCA, L CA, GP180, EC:3.1.3.48, B220

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  APC-Cyanine7
Size: 

-/ -

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经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

AY7-98117 targets CD45 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms LCA, L CA, GP180, EC:3.1.3.48, B220
Gene Symbol CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_3672366
Conjugate APC-Cyanine7 Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 778 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

实验方案

Product Specific Protocols
FC protocol for APC-Cyanine7 CD45 antibody AY7-98117Download protocol
Standard Protocols
Click here to view our Standard Protocols