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APC Anti-Human CD45 Rabbit Recombinant Antibody

CD45 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

241670E10

Cat no : APC-98117

Print datasheet

Synonyms

LCA, L CA, GP180, EC:3.1.3.48, B220

验证数据展示

  • FC experiment of human peripheral blood leukocytes using APC-98117

    FC experiment of human peripheral blood leukocytes using APC-98117

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul APC Anti-Human CD45 Rabbit Recombinant Antibody (APC-98117, Clone: 241670E10) or APC Rabbit IgG Isotype Control Recombinant Antibody (APC-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using APC-98117

    FC experiment of human peripheral blood leukocytes using APC-98117

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul APC Anti-Human CD45 Rabbit Recombinant Antibody (APC-98117, Clone:241670E10) (red) or APC Rabbit IgG Isotype Control Recombinant Antibody (APC-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of APC-98117 is CD45 Fusion Protein expressed in E. coli.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms LCA, L CA, GP180, EC:3.1.3.48, B220
Gene Symbol CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

实验方案

Product Specific Protocols
FC protocol for APC CD45 antibody APC-98117Download protocol
Standard Protocols
Click here to view our Standard Protocols