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验证数据展示

  • FC experiment of human PBMCs using 65226-1-Ig

    FC experiment of human PBMCs using 65226-1-Ig

    1X10^6 human PBMCs were surface co-stained with PE Anti-human CD3, FITC Anti-human CD45RO, and 0.2 ug Anti-Human CD45RA (65226-1-Ig, Clone: F8-11-13) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG1 (KFA023) or Mouse IgG1 Isotype Control. Cells were not fixed. CD3+ lymphocytes were gated.

  • FC experiment of human PBMCs using 65226-1-Ig

    FC experiment of human PBMCs using 65226-1-Ig

    1X10^6 human PBMCs were surface co-stained with PE Anti-human CD3 and 0.2 ug Anti-Human CD45RA (65226-1-Ig, Clone: F8-11-13) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG1 (KFA023) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. CD3+ lymphocytes were gated.

  • IHC staining of human tonsillitis using 65226-1-Ig

    IHC staining of human tonsillitis using 65226-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 65226-1-Ig (CD45RA antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD45 antibody included
  • IF Staining of human tonsillitis using 65226-1-Ig

    IF Staining of human tonsillitis using 65226-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD45RA antibody (65226-1-Ig, Clone: F8-11-13 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Anti-Human CD45RA (F8-11-13)

CD45RA Monoclonal Antibody for IHC, IF-P, FC
Cat No. 65226-1-Ig

产品说明书

CloneNo. F8-11-13

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human

应用

IHC, IF-P, FC

别名

CD45, B220, L CA, GP180, F8-11-13

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:1000-1:4000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65226-1-Ig targets CD45RA in IHC, IF-P, FC applications and shows reactivity with human samples.

经测试应用 IHC, IF-P, FC Application Description
经测试反应性 human
免疫原 Human T lymphocytes 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, C
别名 CD45, B220, L CA, GP180, F8-11-13
计算分子量 1304 aa, 147 kDa
GenBank蛋白编号BC014239
基因名称 CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_3084907
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP08575
储存缓冲液 PBS with 0.09% sodium azide.
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

实验方案

Product Specific Protocols
IHC protocol for CD45RA antibody 65226-1-IgDownload protocol
IF protocol for CD45RA antibody 65226-1-IgDownload protocol
FC protocol for CD45RA antibody 65226-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols