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Anti-Human CD45RA (F8-11-13)

CD45RA Monoclonal Antibody for IF-P, FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

IF-P, FC

Conjugate

Unconjugated

CloneNo.

F8-11-13

Cat no : 65226-1-Ig

Print datasheet

Synonyms

CD45, B220, L CA, GP180, F8-11-13

验证数据展示

  • FC experiment of human PBMCs using 65226-1-Ig

    FC experiment of human PBMCs using 65226-1-Ig

    1X10^6 human PBMCs were surface co-stained with PE Anti-human CD3, FITC Anti-human CD45RO, and 0.2 ug Anti-Human CD45RA (65226-1-Ig, Clone: F8-11-13) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG1 (KFA023) or Mouse IgG1 Isotype Control. Cells were not fixed. CD3+ lymphocytes were gated.

  • FC experiment of human PBMCs using 65226-1-Ig

    FC experiment of human PBMCs using 65226-1-Ig

    1X10^6 human PBMCs were surface co-stained with PE Anti-human CD3 and 0.2 ug Anti-Human CD45RA (65226-1-Ig, Clone: F8-11-13) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG1 (KFA023) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. CD3+ lymphocytes were gated.

  • IF Staining of human tonsillitis using 65226-1-Ig

    IF Staining of human tonsillitis using 65226-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD45RA antibody (65226-1-Ig, Clone: F8-11-13 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:1000-1:4000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65226-1-Ig targets CD45RA in IF-P, FC applications and shows reactivity with human samples.

Tested Applications IF-P, FC Application Description
Tested Reactivity human
Immunogen Human T lymphocytes 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms CD45, B220, L CA, GP180, F8-11-13
Calculated Molecular Weight 1304 aa, 147 kDa
GenBank Accession NumberBC014239
Gene Symbol CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08575
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

实验方案

Product Specific Protocols
IF protocol for CD45RA antibody 65226-1-IgDownload protocol
FC protocol for CD45RA antibody 65226-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols