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Anti-Human CD64 (10.1)

CD64 Monoclonal Antibody for FC, ELISA

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC, ELISA

Conjugate

Unconjugated

CloneNo.

10.1

Cat no : 65253-1-Ig

Print datasheet

Synonyms

CD64, CD64A, Fc gamma RI, Fc gamma RIA, FCG1, FcgammaRIa, FCGR1, FCGR1A, FCRI, FLJ18345, IGFR1, IgG Fc receptor I

验证数据展示

  • FC experiment of human PBMCs using 65253-1-Ig

    FC experiment of human PBMCs using 65253-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD64 (65253-1-Ig, Clone:10.1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or CoraLite®488-Conjugated Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated.

  • FC experiment of human PBMCs using 65253-1-Ig

    FC experiment of human PBMCs using 65253-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD64 (65253-1-Ig, Clone:10.1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or CoraLite®488-Conjugated Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65253-1-Ig targets CD64 in FC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, ELISA Application Description
Tested Reactivity Human
Immunogen Human rheumatoid synovial fluid cells and fibronectin-purified monocytes. 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name Fc fragment of IgG, high affinity Ia, receptor (CD64)
Synonyms CD64, CD64A, Fc gamma RI, Fc gamma RIA, FCG1, FcgammaRIa, FCGR1, FCGR1A, FCRI, FLJ18345, IGFR1, IgG Fc receptor I
Calculated Molecular Weight 374 aa, 43 kDa
GenBank Accession NumberBC032634
Gene Symbol FCGR1A
Gene ID (NCBI) 2209
ENSEMBL Gene IDENSG00000150337
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP12314
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.

实验方案

Product Specific Protocols
FC protocol for CD64 antibody 65253-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols