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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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验证数据展示

  • FC experiment of human PBMCs using 65253-1-Ig

    FC experiment of human PBMCs using 65253-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD64 (65253-1-Ig, Clone:10.1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or CoraLite®488-Conjugated Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated.

  • FC experiment of human PBMCs using 65253-1-Ig

    FC experiment of human PBMCs using 65253-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD64 (65253-1-Ig, Clone:10.1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or CoraLite®488-Conjugated Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated.

Anti-Human CD64 (10.1)

CD64 Monoclonal Antibody for FC, ELISA
Cat No. 65253-1-Ig

产品说明书

CloneNo. 10.1

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human

应用

FC, ELISA

别名

CD64, CD64A, Fc gamma RI, Fc gamma RIA, FCG1, FcgammaRIa, FCGR1, FCGR1A, FCRI, FLJ18345, IGFR1, IgG Fc receptor I

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65253-1-Ig targets CD64 in FC, ELISA applications and shows reactivity with Human samples.

经测试应用 FC, ELISA Application Description
经测试反应性 Human
免疫原 Human rheumatoid synovial fluid cells and fibronectin-purified monocytes. 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 Fc fragment of IgG, high affinity Ia, receptor (CD64)
别名 CD64, CD64A, Fc gamma RI, Fc gamma RIA, FCG1, FcgammaRIa, FCGR1, FCGR1A, FCRI, FLJ18345, IGFR1, IgG Fc receptor I
计算分子量 374 aa, 43 kDa
GenBank蛋白编号BC032634
基因名称 FCGR1A
Gene ID (NCBI) 2209
ENSEMBL Gene IDENSG00000150337
RRIDAB_3084911
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP12314
储存缓冲液 PBS with 0.09% sodium azide.
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.

实验方案

Product Specific Protocols
FC protocol for CD64 antibody 65253-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols