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CD70 Monoclonal antibody

CD70 Monoclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A11A10

Cat No : 67749-1-Ig

Print datasheet

Synonyms

CD27 L, CD27 ligand, CD27L, CD27LG, CD70, CD70 antigen, CD70 molecule, TNFSF7

验证数据展示

  • WB analysis using 67749-1-Ig

    WB analysis using 67749-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67749-1-Ig (CD70 antibody) at dilution of 1:1000, 2000, 4000 and 8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 67749-1-Ig

    IHC staining of human tonsillitis using 67749-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67749-1-Ig (CD70 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 67749-1-Ig

    IHC staining of human tonsillitis using 67749-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67749-1-Ig (CD70 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 67749-1-Ig

    IF Staining of human tonsillitis using 67749-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD70 antibody (67749-1-Ig, Clone: 1A11A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inTHP-1 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

67749-1-Ig targets CD70 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF-P, IHC, WB, ELISA
Cited ApplicationsIHC
Tested Reactivity Human
Cited Reactivityhuman
Immunogen CD70 fusion protein Ag28070 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD70 molecule
Synonyms CD27 L, CD27 ligand, CD27L, CD27LG, CD70, CD70 antigen, CD70 molecule, TNFSF7
Calculated Molecular Weight 21 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC000725
Gene Symbol CD70
Gene ID (NCBI) 970
RRIDAB_2918518
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP32970
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CD70 antibody 67749-1-IgDownload protocol
IHC protocol for CD70 antibody 67749-1-IgDownload protocol
IF protocol for CD70 antibody 67749-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Int J Cancer

Special issue "The advance of solid tumor research in China": Multi-omics analysis based on 1311 clear cell renal cell carcinoma samples identifies a glycolysis signature associated with prognosis and treatment response

Authors - Xi Tian
View Article