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CD80/B7-1 Monoclonal antibody, PBS Only

CD80/B7-1 Monoclonal Antibody for WB,IHC,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB,IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1E2F10

Cat No : 66406-1-PBS

Print datasheet

Synonyms

Activation B7 1 antigen, B7, B7 1, B7-1, BB1, CD28LG, CD28LG1, CD80, CD80 molecule, CD80/B7-1, CTLA 4 counter receptor B7.1, LAB7

验证数据展示

  • WB analysis of Raji using 66406-1-Ig (same clone as 66406-1-PBS)

    WB analysis of Raji using 66406-1-Ig (same clone as 66406-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80 / B7-1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66406-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66406-1-Ig (same clone as 66406-1-PBS)

    IHC staining of human tonsillitis using 66406-1-Ig (same clone as 66406-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66406-1-Ig (CD80 / B7-1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66406-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66406-1-Ig (same clone as 66406-1-PBS)

    IHC staining of human tonsillitis using 66406-1-Ig (same clone as 66406-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66406-1-Ig (CD80 / B7-1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66406-1-PBS in a different storage buffer formulation.

产品信息

66406-1-PBS targets CD80/B7-1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Applications WB,IHC,ELISA Application Description
Tested Reactivity human, mouse, pig
Immunogen CD80/B7-1 fusion protein Ag5615 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD80 molecule
Synonyms Activation B7 1 antigen, B7, B7 1, B7-1, BB1, CD28LG, CD28LG1, CD80, CD80 molecule, CD80/B7-1, CTLA 4 counter receptor B7.1, LAB7
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC042665
Gene Symbol CD80
Gene ID (NCBI) 941
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP33681
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)