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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66866-1-Ig (same clone as 66866-1-PBS)

    WB analysis using 66866-1-Ig (same clone as 66866-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66866-1-Ig (CD81 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66866-1-PBS in a different storage buffer formulation.

  • WB analysis of HCT 116 using 66866-1-Ig (same clone as 66866-1-PBS)

    WB analysis of HCT 116 using 66866-1-Ig (same clone as 66866-1-PBS)

    WB result of CD81 antibody (66866-1-Ig; 1:3000; room temperature for 1.5 hours) with wild-type and CD81 knockout HCT 116 cells. This data was developed using the same antibody clone with 66866-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66866-1-Ig (same clone as 66866-1-PBS)

    IHC staining of human tonsillitis using 66866-1-Ig (same clone as 66866-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66866-1-Ig (CD81 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66866-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66866-1-Ig (same clone as 66866-1-PBS)

    IHC staining of human tonsillitis using 66866-1-Ig (same clone as 66866-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66866-1-Ig (CD81 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66866-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66866-1-Ig (same clone as 66866-1-PBS)

    IF Staining of human tonsillitis using 66866-1-Ig (same clone as 66866-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD81 antibody (66866-1-Ig, Clone: 1G2C6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66866-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66866-1-Ig (same clone as 66866-1-PBS)

    IF Staining of human tonsillitis using 66866-1-Ig (same clone as 66866-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD81 antibody (66866-1-Ig, Clone: 1G2C6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66866-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CD81 Monoclonal antibody, PBS Only

CD81 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66866-1-PBS

Print datasheet

CloneNo. 1G2C6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

TAPA1, S5.7, CD81 molecule, CD81 antigen, 26 kDa cell surface protein TAPA-1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

66866-1-PBS targets CD81 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human
Immunogen CD81 fusion protein Ag27298 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD81 molecule
Synonyms TAPA1, S5.7, CD81 molecule, CD81 antigen, 26 kDa cell surface protein TAPA-1
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC002978
Gene Symbol CD81
Gene ID (NCBI) 975
ENSEMBL Gene IDENSG00000110651
RRIDAB_2882203
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP60033
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).