CD86 Polyclonal antibody
CD86 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, ELISA and More (2)
Conjugate
Unconjugated
201
验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, Raji cells, Ramos cells, RAW 264.7 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13395-1-AP targets CD86 in WB, IF, ELISA, Cell treatment applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IF, Cell treatment |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, rabbit |
| Immunogen | CD86 fusion protein Ag4063 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | CD86 molecule |
| Synonyms | CD28LG2, BU63, B70, B7 2, Activation B7-2 antigen |
| Calculated Molecular Weight | 329 aa, 38 kDa |
| Observed Molecular Weight | 60-80 kDa |
| GenBank Accession Number | BC040261 |
| Gene Symbol | CD86 |
| Gene ID (NCBI) | 942 |
| ENSEMBL Gene ID | ENSG00000114013 |
| RRID | AB_2074882 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42081 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. This antibody detects a band of approximately 65-70 kDa, consistent with glycosylated form of CD86 (PMID: 8592066, 21849678).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD86 antibody 13395-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
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