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验证数据展示

  • WB analysis of HeLa using 60232-1-Ig (same clone as 60232-1-PBS)

    WB analysis of HeLa using 60232-1-Ig (same clone as 60232-1-PBS)

    WB result of CD9 antibody (60232-1-Ig, 1:2000) with si-Control and si-CD9 transfected HeLa cells. This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • WB analysis using 60232-1-Ig (same clone as 60232-1-PBS)

    WB analysis using 60232-1-Ig (same clone as 60232-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60232-1-Ig (CD9 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

    IHC staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD9 antibody included
  • IHC staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

    IHC staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD9 antibody included
  • IF Staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    IF Staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    IF Staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

    IHC staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD9 antibody included
  • IF Staining of MCF-7 using 60232-1-Ig (same clone as 60232-1-PBS)

    IF Staining of MCF-7 using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CD9 Monoclonal antibody, PBS Only

CD9 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 60232-1-PBS

产品说明书

CloneNo. 4H7B9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD9 Ready-To-Use IHC Kit. CD9 primary antibody included.

产品信息

60232-1-PBS targets CD9 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原 CD9 fusion protein Ag14529 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CD9 molecule
别名 CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9
计算分子量 228 aa, 25 kDa
观测分子量 23-27 kDa
GenBank蛋白编号BC011988
基因名称 CD9
Gene ID (NCBI) 928
RRIDAB_11232215
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP21926
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.