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验证数据展示

  • FC experiment of human peripheral blood platelets using PE-65070

    FC experiment of human peripheral blood platelets using PE-65070

    1X10^6 human peripheral blood platelets were surface stained with 10 ul PE Anti-Human CD9 (PE-65070, Clone:MM2/57) (red) or PE-mouse IgG2b isotype control (black). Cells were not fixed.

PE Anti-Human CD9 (MM2/57)

CD9 Monoclonal Antibody for FC
Cat No. PE-65070

产品说明书

CloneNo. MM2/57

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

FC

别名

5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, Cell and organelle markers, DRAP 27, Exosome marker, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  PE
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman peripheral blood platelets
Planning an IHC experiment? We recommend our IHCeasy CD9 Ready-To-Use IHC Kit. CD9 primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65070 targets CD9 in FC applications and shows reactivity with Human samples.

经测试应用 FC Application Description
经测试反应性 Human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 CD9 molecule
别名 5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, Cell and organelle markers, DRAP 27, Exosome marker, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29
计算分子量 228 aa, 25 kDa
GenBank蛋白编号BC011988
基因名称 CD9
Gene ID (NCBI) 928
RRIDAB_2883867
偶联类型 PE Fluorescent Dye
最大激发/发射波长496 nm, 565 nm / 578 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP21926
储存缓冲液 PBS with 0.1% sodium azide and a stabilizer , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD9, also known as Tspan-29, p24 or MIC3, is a member of the tetraspanin superfamily (PMID: 1879540). It is expressed on a large variety of hematopoietic and non-hematopoietic cells, such as stromal cells, megakaryocytes, platelets, B and T lymphocytes, dendritic cells, endothelial cells, mast cells, eosinophils, and basophils (PMID: 30356731). CD9 interacts with the integrin family and other membrane proteins, and is involved in cell adhesion, cell motility and tumor metastasis (PMID: 8478605; 21428940; 25805926). Expression of CD9 enhances membrane fusion between muscle cells and support myotube maintenance (PMID:10459022). On oocytes, CD9 is hypothesized to play a role in fertilization of mammals (PMID: 25536312).

实验方案

Product Specific Protocols
FC protocol for PE CD9 antibody PE-65070Download protocol
Standard Protocols
Click here to view our Standard Protocols