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CDC25C Monoclonal antibody, PBS Only

CDC25C Monoclonal Antibody for Indirect ELISA, WB

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

2B6B12

Cat no : 66912-1-PBS

Print datasheet

Synonyms

M-phase inducer phosphatase 3, M phase inducer phosphatase 3, EC:3.1.3.48, CDC25, 2B6B12

验证数据展示

  • WB analysis using 66912-1-Ig (same clone as 66912-1-PBS)

    WB analysis using 66912-1-Ig (same clone as 66912-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66912-1-PBS in a different storage buffer formulation.

  • WB analysis using 66912-1-Ig (same clone as 66912-1-PBS)

    WB analysis using 66912-1-Ig (same clone as 66912-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66912-1-PBS in a different storage buffer formulation.

产品信息

66912-1-PBS targets CDC25C in Indirect ELISA, WB applications and shows reactivity with rat, mouse, human samples.

Tested Applications Indirect ELISA, WB Application Description
Tested Reactivity rat, mouse, human
Immunogen CDC25C fusion protein Ag10240 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cell division cycle 25 homolog C (S. pombe)
Synonyms M-phase inducer phosphatase 3, M phase inducer phosphatase 3, EC:3.1.3.48, CDC25, 2B6B12
Calculated Molecular Weight 473 aa, 53 kDa
Observed Molecular Weight 46-53 kDa
GenBank Accession NumberBC019089
Gene Symbol CDC25C
Gene ID (NCBI) 995
RRIDAB_2882239
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30307
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The cell division cycle 25 (CDC25) family of proteins are highly conserved dual specificity phosphatases that activate cyclin-dependent kinase (CDK) complexes, which in turn regulate progression through the cell division cycle. CDC25C (cell division cycle 25 homolog C) is one of the 3 isoforms of CDC25 in mammalian cells. It is present in the cytoplasm of asynchronously growing human cells (PMID:10330186). And it may be phosphorylated by its own substrate, an active cdc2-cyclin B complex, to create an autoactivation loop (PMID:7937793). It can be hyperphosphorylated to get the active form of 70 kDa (PMID:18296513). CDC25C has some isofroms with molecular mass of 46-53 kDa.