验证数据展示

WB analysis of HeLa using 19117-1-AP
WB result of CDK6 antibody (19117-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK6 transfected HeLa cells.
WB analysis using 19117-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19117-1-AP (CDK6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 19117-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19117-1-AP (CDK6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 19117-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19117-1-AP (CDK6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CDK6 antibody included
IHC staining of human lung cancer using 19117-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19117-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CDK6 antibody included
IF Staining of HeLa using 19117-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK6 antibody (19117-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IP experiment of Jurkat using 19117-1-AP
IP result of anti-CDK6 (IP:19117-1-AP, 4ug; Detection:19117-1-AP 1:1000) with Jurkat cells lysate 1960 ug.
FC experiment of HeLa using 19117-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDK6 (19117-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

CDK6 Polyclonal antibody

CDK6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 19117-1-AP

产品说明书

引用文献(21)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

CDKN6, Cell division protein kinase 6, Cyclin dependent kinase 6, Cyclin-dependent kinase 6, EC:2.7.11.22

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (21)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HeLa cells, HEK-293 cells, Jurkat cells, HepG2 cells, K-562 cells, HSC-T6 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human lung cancer tissue, human gliomas tissue, human endometrial cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy CDK6 Ready-To-Use IHC Kit. CDK6 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 18 publications below
IHC	See 1 publications below

产品信息

19117-1-AP targets CDK6 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat
免疫原	CatNo: Ag5600 Product name: Recombinant human CDK6 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-326 aa of BC027989 Sequence: MEKDGLCRADQQYECVAEIGEGAYGKVFKARDLKNGGRFVALKRVRVQTGEEGMPLSTIREVAVLRHLETFEHPNVVRLFDVCTVSRTDRETKLTLVFEHVDQDLTTYLDKVPEPGVPTETIKDMMFQLLRGLDFLHSHRVVHRDLKPQNILVTSSGQIKLADFGLARIYSFQMALTSVVVTLWYRAPEVLLQSSYATPVDLWSVGCIFAEMFRRKPLFRGSSDVDQLGKILDVIGLPGEEDWPRDVALPRQAFHSKSAQPIEKFVTDIDELGKDLLLKCLTFNPAKRISAYSALSHPYFQDLERCKENLDSHLPPSQNTSELNTA 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	cyclin-dependent kinase 6
别名	CDKN6, Cell division protein kinase 6, Cyclin dependent kinase 6, Cyclin-dependent kinase 6, EC:2.7.11.22
计算分子量	326 aa, 36 kDa
观测分子量	36-40 kDa
GenBank蛋白编号	BC027989
基因名称	CDK6
Gene ID (NCBI)	1021
RRID	AB_10640575
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q00534
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739).

实验方案

Product Specific Protocols
FC protocol for CDK6 antibody 19117-1-AP	Download protocol
IF protocol for CDK6 antibody 19117-1-AP	Download protocol
IHC protocol for CDK6 antibody 19117-1-AP	Download protocol
IP protocol for CDK6 antibody 19117-1-AP	Download protocol
WB protocol for CDK6 antibody 19117-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Mol Cell CDK-independent role of D-type cyclins in regulating DNA mismatch repair Authors - Gergely Rona View Article
human	WB	Theranostics Estrogen-induced circRNA, circPGR, functions as a ceRNA to promote estrogen receptor-positive breast cancer cell growth by regulating cell cycle-related genes. Authors - Lei Wang View Article
mouse	WB	Nat Cardiovasc Res α-Ketoglutarate promotes cardiomyocyte proliferation and heart regeneration after myocardial infarction Authors - Yu Shi View Article
human	WB	Cell Death Dis GLI1 reduces drug sensitivity by regulating cell cycle through PI3K/AKT/GSK3/CDK pathway in acute myeloid leukemia. Authors - Cheng Zhou View Article
mouse	WB	Oxid Med Cell Longev P16INK4a Regulates ROS-Related Autophagy and CDK4/6-Mediated Proliferation: A New Target of Myocardial Regeneration Therapy Authors - Jiateng Sun View Article
rat	WB	Front Pharmacol Methamphetamine exposure drives cell cycle exit and aberrant differentiation in rat hippocampal-derived neurospheres Authors - Shaomin Wang View Article

验证数据展示

WB analysis of HeLa using 19117-1-AP

WB analysis using 19117-1-AP

WB analysis using 19117-1-AP

IHC staining of human lung cancer using 19117-1-AP

IHC staining of human lung cancer using 19117-1-AP

IF Staining of HeLa using 19117-1-AP

IP experiment of Jurkat using 19117-1-AP

FC experiment of HeLa using 19117-1-AP

CDK6 Polyclonal antibody

CDK6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

CDK-independent role of D-type cyclins in regulating DNA mismatch repair

Estrogen-induced circRNA, circPGR, functions as a ceRNA to promote estrogen receptor-positive breast cancer cell growth by regulating cell cycle-related genes.

α-Ketoglutarate promotes cardiomyocyte proliferation and heart regeneration after myocardial infarction

GLI1 reduces drug sensitivity by regulating cell cycle through PI3K/AKT/GSK3/CDK pathway in acute myeloid leukemia.

P16INK4a Regulates ROS-Related Autophagy and CDK4/6-Mediated Proliferation: A New Target of Myocardial Regeneration Therapy

Methamphetamine exposure drives cell cycle exit and aberrant differentiation in rat hippocampal-derived neurospheres

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