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  • Featured Product
  • KD/KO Validated

CDK6 Polyclonal antibody

CDK6 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 19117-1-AP

Print datasheet

Synonyms

CDK6, Cell division protein kinase 6, cyclin dependent kinase 6, PLSTIRE

验证数据展示

  • WB analysis of HeLa using 19117-1-AP

    WB analysis of HeLa using 19117-1-AP

    WB result of CDK6 antibody (19117-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK6 transfected HeLa cells.

  • WB analysis using 19117-1-AP

    WB analysis using 19117-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19117-1-AP (CDK6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 19117-1-AP

    IHC staining of human lung cancer using 19117-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19117-1-AP (CDK6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 19117-1-AP

    IHC staining of human lung cancer using 19117-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19117-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 19117-1-AP

    IF Staining of HeLa using 19117-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK6 antibody (19117-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IP experiment of Jurkat using 19117-1-AP

    IP experiment of Jurkat using 19117-1-AP

    IP result of anti-CDK6 (IP:19117-1-AP, 4ug; Detection:19117-1-AP 1:700) with Jurkat cells lysate 3200ug.

  • FC experiment of HeLa using 19117-1-AP

    FC experiment of HeLa using 19117-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDK6 (19117-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, HepG2 cells, K-562 cells, HSC-T6 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman lung cancer tissue, human gliomas tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 13 publications below

产品信息

19117-1-AP targets CDK6 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA
Cited Applications WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CDK6 fusion protein Ag5600 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin-dependent kinase 6
Synonyms CDK6, Cell division protein kinase 6, cyclin dependent kinase 6, PLSTIRE
Calculated Molecular Weight 326 aa, 36 kDa
Observed Molecular Weight 36-40 kDa
GenBank Accession NumberBC027989
Gene Symbol CDK6
Gene ID (NCBI) 1021
RRIDAB_10640575
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00534
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739).

实验方案

Product Specific Protocols
WB protocol for CDK6 antibody 19117-1-APDownload protocol
IHC protocol for CDK6 antibody 19117-1-APDownload protocol
IF protocol for CDK6 antibody 19117-1-APDownload protocol
IP protocol for CDK6 antibody 19117-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Theranostics

Estrogen-induced circRNA, circPGR, functions as a ceRNA to promote estrogen receptor-positive breast cancer cell growth by regulating cell cycle-related genes.

Authors - Lei Wang
View Article
humanWB

Cancers (Basel)

A Patient-Derived Xenograft Model of Dedifferentiated Endometrial Carcinoma: A Proof-of-Concept Study for the Identification of New Molecularly Informed Treatment Approaches.

Authors - Chiao-Yun Lin
View Article
humanWB

Oncotarget

High expression of N-myc (and STAT) interactor predicts poor prognosis and promotes tumor growth in human glioblastoma.

Authors - Delong Meng
View Article
humanWB

Front Pharmacol

Oxymatrine Inhibits Renal Cell Carcinoma Progression by Suppressing β-Catenin Expression.

Authors - Yinshan Jin
View Article
humanWB

Cancer Biother Radiopharm

γ-Glutamylcyclotransferase Knockdown Inhibits Growth of Lung Cancer Cells Through G0/G1 Phase Arrest.

Authors - Zhifeng Lin
View Article
humanWB

Cancer Med

δ-Catenin regulates proliferation and apoptosis in renal cell carcinoma via promoting β-catenin nuclear localization and activating its downstream target genes.

Authors - Lincheng Ju
View Article