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验证数据展示

  • WB analysis of Jurkat using 25235-1-AP

    WB analysis of Jurkat using 25235-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25235-1-AP (CEP110 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of MDCK using 25235-1-AP

    IF Staining of MDCK using 25235-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 25235-1-AP (CEP110 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

CEP110 Polyclonal antibody

CEP110 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25235-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, Canine

应用

WB, IF/ICC, ELISA

别名

110 kDa centrosomal protein, Centriolin, Centrosomal protein 1, centrosomal protein 110kDa, CEP1, CEP110, FAN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells
Positive IF/ICC detected inMDCK cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25235-1-AP targets CEP110 in WB, IF/ICC, ELISA applications and shows reactivity with human, Canine samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, Canine
免疫原 CEP110 fusion protein Ag18907 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 centrosomal protein 110kDa
别名 110 kDa centrosomal protein, Centriolin, Centrosomal protein 1, centrosomal protein 110kDa, CEP1, CEP110, FAN
计算分子量 2325 aa, 269 kDa
GenBank蛋白编号BC137284
基因名称 CEP110
Gene ID (NCBI) 11064
RRIDAB_2879979
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z7A1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CEP110 antibody 25235-1-APDownload protocol
IF protocol for CEP110 antibody 25235-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols