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CHAC1 Polyclonal antibody

CHAC1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15207-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, hamster and More (2)

应用

WB, IHC, ELISA and More (3)

Glutathione-specific gamma-glutamylcyclotransferase 1, Gamma-GCG 1, EC:4.3.2.7, Cation transport regulator-like protein 1, Botch

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, A549 cells, CHO cells, U-251 cells, C6 cells, RAW 264.7 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15207-1-AP targets CHAC1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat, hamster
文献引用反应性human, mouse, rat, chicken, yeast
免疫原 CHAC1 fusion protein Ag7360 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ChaC, cation transport regulator homolog 1 (E. coli)
别名 Glutathione-specific gamma-glutamylcyclotransferase 1, Gamma-GCG 1, EC:4.3.2.7, Cation transport regulator-like protein 1, Botch
计算分子量 24 kDa
观测分子量 38 kDa
GenBank蛋白编号BC001847
基因名称 CHAC1
Gene ID (NCBI) 79094
RRIDAB_2878118
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BUX1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHAC1(Cation transport regulator-like protein 1) was identified in a co-regulated group of genes enriched for components of the ATF4 (activating transcription factor 4) arm of the unfolded protein response pathway. CHAC1 is a proapoptotic ER stress protein downstream of the pancreatic EIF2α kinase-ATF4 pathway that appears to be important for human physiology and disease(PMID: 19109178). In addition, the study found that high CHAC1 expression is associated with a bad prognosis hinting that CHAC1 may have a possible prognostic significance in breast cancer(PMID: 35930144). The predicted molecular weight of CHAC1 is 24 kDa, and the molecular weight detected by Proteintech is 38 kDa.

实验方案

Product Specific Protocols
WB protocol for CHAC1 antibody 15207-1-APDownload protocol
IHC protocol for CHAC1 antibody 15207-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Proc Natl Acad Sci U S A

Consequences of poly(ethylene oxide) and poloxamer P188 on transcription in healthy and stressed myoblasts

Authors - Adelyn A Crabtree
mouseWB

Cell Mol Gastroenterol Hepatol

Hepatic TGFβr1 Deficiency Attenuates Lipopolysaccharide/D-Galactosamine-Induced Acute Liver Failure Through Inhibiting GSK3β-Nrf2-Mediated Hepatocyte Apoptosis and Ferroptosis.

Authors - Sha Huang
humanWB

Free Radic Biol Med

High-fat diet aggravates colitis-associated carcinogenesis by evading ferroptosis in the ER stress-mediated pathway.

Authors - Xiaoli Zhang
humanWB

Lung

ETS1 Ameliorates Hyperoxia-Induced Bronchopulmonary Dysplasia in Mice by Activating Nrf2/HO-1 Mediated Ferroptosis

Authors - Min Yang
humanWB

Front Oncol

Development and validation of an endoplasmic reticulum stress-related molecular prognostic model for breast cancer

Authors - Pengyu Fan
humanIHC

J Cell Mol Med

Ferroptosis-related gene NOX4, CHAC1 and HIF1A are valid biomarkers for stomach adenocarcinoma.

Authors - Ruoxi Xiao
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