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CHMP1A Polyclonal antibody

CHMP1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15761-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

KIAA0047, hVps46-1, hVps46 1, Chromatin-modifying protein 1a, chromatin modifying protein 1A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, mouse lung tissue, HeLa cells, A431 cells, mouse kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse brain tissue, human lung tissue, mouse testis tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CHMP1A Ready-To-Use IHC Kit. CHMP1A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15761-1-AP targets CHMP1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 CHMP1A fusion protein Ag8389 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromatin modifying protein 1A
别名 KIAA0047, hVps46-1, hVps46 1, Chromatin-modifying protein 1a, chromatin modifying protein 1A
计算分子量 196 aa, 22 kDa
观测分子量 33 kDa, 25 kDa
GenBank蛋白编号BC007527
基因名称 CHMP1A
Gene ID (NCBI) 5119
RRIDAB_2229399
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HD42
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Charged multivesicular body protein 1A (CHMP1A), also known as chromatin modifying protein 1A, is a member of the ESCRT-III (endosomal sorting complex required for transport III) complex which is involved in degradation of internalized transmembrane receptor proteins and formation of multivesicular bodies (MVBs) (PMID: 16730941). CHMP1A has both nuclear and cytoplasmic distributions (PMID: 11559748; 11559747). Subcellular localization of CHMP1A seems to vary depending on the cell type (PMID: 23023333). CHMP1A is required for MVBs formation and has a role in stable gene silencing within the nucleus. Besides, the tumor suppressor role of CHMP1A has also been reported (PMID: 18787405; 22261332).

实验方案

Product Specific Protocols
WB protocol for CHMP1A antibody 15761-1-APDownload protocol
IHC protocol for CHMP1A antibody 15761-1-APDownload protocol
IF protocol for CHMP1A antibody 15761-1-APDownload protocol
IP protocol for CHMP1A antibody 15761-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Cell Biol

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Authors - Vincent Mercier
humanWB

J Extracell Vesicles

Accessory ESCRT-III proteins are conserved and selective regulators of Rab11a-exosome formation

Authors - Pauline P Marie
mouseWB,IF

Nat Commun

A single genetic locus controls both expression of DPEP1/CHMP1A and kidney disease development via ferroptosis.

Authors - Yuting Guan
  • KO Validated
mouseWB

Nat Commun

Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair.

Authors - Luana L Scheffer
human,mouseIHC

Cell Rep

The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles.

Authors - Michael E Coulter
  • KO Validated
humanWB

Cell Rep

Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.

Authors - Inna Goliand
  • KD Validated
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