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CHMP1A Polyclonal antibody
CHMP1A Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC, IHC, IP, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse lung tissue, HeLa cells, A431 cells, mouse kidney tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse brain tissue, human lung tissue, mouse testis tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy CHMP1A Ready-To-Use IHC Kit. CHMP1A primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15761-1-AP targets CHMP1A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC, IHC, IP, WB,ELISA |
| Cited Applications | IF, IHC, WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | CHMP1A fusion protein Ag8389 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | chromatin modifying protein 1A |
| Synonyms | CHMP1, CHMP1A, chromatin modifying protein 1A, hVps46 1, KIAA0047, PCOLN3, PRSM1, Vps46 1 |
| Calculated Molecular Weight | 196 aa, 22 kDa |
| Observed Molecular Weight | 33 kDa, 25 kDa |
| GenBank Accession Number | BC007527 |
| Gene Symbol | CHMP1A |
| Gene ID (NCBI) | 5119 |
| RRID | AB_2229399 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HD42 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Charged multivesicular body protein 1A (CHMP1A), also known as chromatin modifying protein 1A, is a member of the ESCRT-III (endosomal sorting complex required for transport III) complex which is involved in degradation of internalized transmembrane receptor proteins and formation of multivesicular bodies (MVBs) (PMID: 16730941). CHMP1A has both nuclear and cytoplasmic distributions (PMID: 11559748; 11559747). Subcellular localization of CHMP1A seems to vary depending on the cell type (PMID: 23023333). CHMP1A is required for MVBs formation and has a role in stable gene silencing within the nucleus. Besides, the tumor suppressor role of CHMP1A has also been reported (PMID: 18787405; 22261332).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CHMP1A antibody 15761-1-AP | Download protocol |
| IHC protocol for CHMP1A antibody 15761-1-AP | Download protocol |
| IF protocol for CHMP1A antibody 15761-1-AP | Download protocol |
| IP protocol for CHMP1A antibody 15761-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation. | ||
J Extracell Vesicles Accessory ESCRT-III proteins are conserved and selective regulators of Rab11a-exosome formation | ||
Nat Commun A single genetic locus controls both expression of DPEP1/CHMP1A and kidney disease development via ferroptosis.
| ||
Nat Commun Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair. | ||
Cell Rep The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles.
| ||
Cell Rep Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.
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