Claudin 18 Polyclonal antibody

Claudin 18 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21126-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

UNQ778/PRO1572, CLDN18, Claudin-18, Claudin18

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse kidney tissue
Positive IP detected inmouse stomach tissue
Positive IHC detected inhuman stomach tissue, human stomach cancer tissue, human ovary tumor tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMKN-45 cells
Planning an IHC experiment? We recommend our IHCeasy Claudin 18 Ready-To-Use IHC Kit. Claudin 18 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21126-1-AP targets Claudin 18 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 Claudin 18 fusion protein Ag15517 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 claudin 18
别名 UNQ778/PRO1572, CLDN18, Claudin-18, Claudin18
计算分子量 261 aa, 28 kDa
观测分子量 28-29 kDa
GenBank蛋白编号BC146668
基因名称 CLDN18
Gene ID (NCBI) 51208
RRIDAB_10733638
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP56856
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Claudin 18 (CLDN18) belongs to the large claudin family of proteins, which are tetraspan transmembrane proteins of tight junctions (PMID: 11585919; 18036336). Claudins exhibit specific patterns of expression in different tissues (PMID: 15447685). CLDN18 is specifically expressed in the stomach and lung. CLDN18 has two alternatively spliced variants, CLDN18.1 and CLDN18.2. CLDN18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas CLDN18.1 is lung specific (PMID: 11585919; 21832145). Altered CLDN18 expression has been reported in various human malignancies, including gastric cancer, lung adenocarcinoma, and pancreatic neoplasm (PMID: 17459057; 22076167; 21832145). This antibody can recognize both CLDN18.1 and CLDN18.2.

实验方案

Product Specific Protocols
WB protocol for Claudin 18 antibody 21126-1-APDownload protocol
IHC protocol for Claudin 18 antibody 21126-1-APDownload protocol
IF protocol for Claudin 18 antibody 21126-1-APDownload protocol
IP protocol for Claudin 18 antibody 21126-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cell Rep Med

Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application

Authors - Xiaoxue Ren
mouseIHC

Ann Transl Med

Inhibition of protein kinase C alpha attenuates lipopolysaccharide-triggered acute lung injury by alleviating the hyperinflammatory response and oxidative stress.

Authors - Yang Chen
mouseWB,IF

Int J Mol Med

Investigating the regulatory role of ORMDL3 in airway barrier dysfunction using in vivo and in vitro models.

Authors - Ruixue Yang
mouseIF

BMC Pulm Med

SPAK-p38 MAPK signal pathway modulates claudin-18 and barrier function of alveolar epithelium after hyperoxic exposure.

Authors - Chih-Hao Shen
ratIF

J Stroke Cerebrovasc Dis

Effect of MicroRNA-126a-3p on Bone Marrow Mesenchymal Stem Cells Repairing Blood-brain Barrier and Nerve Injury after Intracerebral Hemorrhage.

Authors - Chunyan Wang
humanIHC

Cancers (Basel)

Primary Colorectal Tumor Displays Differential Genomic Expression Profiles Associated with Hepatic and Peritoneal Metastases

Authors - Maximiliano Gelli
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