验证数据展示
经过测试的应用
| Positive IP detected in | mouse large intestine tissue |
| Positive IHC detected in | human breast cancer tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 12 publications below |
| IF | See 14 publications below |
产品信息
16456-1-AP targets Claudin 3 in IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IP, IHC, ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, sheep |
| 免疫原 | Claudin 3 fusion protein Ag9411 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | claudin 3 |
| 别名 | C7orf1, claudin 3, Claudin3, CLDN3, CPE R 2, CPE R2, CPE receptor 2, CPETR2, HRVP1, RVP1 |
| 计算分子量 | 220 aa, 23 kDa |
| 观测分子量 | 20-24 kDa |
| GenBank蛋白编号 | BC016056 |
| 基因名称 | Claudin 3 |
| Gene ID (NCBI) | 1365 |
| RRID | AB_10733226 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15551 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Claudin 3 antibody 16456-1-AP | Download protocol |
| IP protocol for Claudin 3 antibody 16456-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging Cell Advanced maternal age causes premature placental senescence and malformation via dysregulated α-Klotho expression in trophoblasts. | ||
EMBO Mol Med Deficiency in intestinal epithelial O-GlcNAcylation predisposes to gut inflammation. | ||
Sci China Life Sci Longitudinal gut fungal alterations and potential fungal biomarkers for the progression of primary liver disease | ||
J Hazard Mater Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles. | ||
Environ Int Translocation of transition metal oxide nanoparticles to breast milk and offspring: The necessity of bridging mother-offspring-integration toxicological assessments. | ||
Biochem Pharmacol Inhibition of S100A9 alleviates neurogenic pulmonary edema after subarachnoid hemorrhage |
