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验证数据展示

  • WB analysis of HEK-293 using 21657-1-AP

    WB analysis of HEK-293 using 21657-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21657-1-AP (CNGA3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 21657-1-AP

    IP experiment of mouse brain using 21657-1-AP

    IP result of anti-CNGA3 (IP:21657-1-AP, 4ug; Detection:21657-1-AP 1:300) with mouse brain tissue lysate 3000ug.

  • IHC staining of mouse eye using 21657-1-AP

    IHC staining of mouse eye using 21657-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21657-1-AP (CNGA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CNGA3 Polyclonal antibody

CNGA3 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 21657-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

ACHM2, CCNC1, CCNCa, CCNCalpha, CNCG3, CNG 3, CNG channel alpha 3, CNG3, CNGA3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21657-1-AP targets CNGA3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 CNGA3 fusion protein Ag16385 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclic nucleotide gated channel alpha 3
别名 ACHM2, CCNC1, CCNCa, CCNCalpha, CNCG3, CNG 3, CNG channel alpha 3, CNG3, CNGA3
计算分子量 694 aa, 79 kDa
观测分子量 98 kDa
GenBank蛋白编号BC096298
基因名称 CNGA3
Gene ID (NCBI) 1261
RRIDAB_10734591
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16281
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CNGA3 is a member of the cyclic nucleotide-gated cation channel protein family which is required for normal vision and olfactory signal transduction. Mutations in CNGA3 gene are associated with achromatopsia (rod monochromacy) and color blindness. Three alternatively spliced transcripts encoding different isoforms have been described. This antibody detects CNGA3 with an apparent molecular weight of 98 kDa which has been reported (PMID: 20378608).

实验方案

Product Specific Protocols
WB protocol for CNGA3 antibody 21657-1-APDownload protocol
IHC protocol for CNGA3 antibody 21657-1-APDownload protocol
IP protocol for CNGA3 antibody 21657-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols